CORE--ANIMAL PRODUCTION

动物生产核心将继续提供选择性繁殖的 以及近交系P和NP（酒精偏好和非酒精偏好）大鼠 作为选择性饲养的HAD和LAD（高和低酒精饮用）大鼠， 实验使用。 这些啮齿类动物将提供给研究人员 由印第安纳州大学ARC支持或附属于ARC， 在其他机构的合作和独立研究人员。为了 为了实现这一目标：1）大鼠的P和NP系将继续 通过宽松的选择程序进行选择性繁殖。 2)P和NP近交系 将通过持续的近亲繁殖来维持每种四个品系。 3)的 选择性繁殖的HAD 1/LAD 1和HAD 2/LAD 2的第二窝和第三窝 将从每一代中产生大鼠品系用于研究。 四、 将继续HAD 1/LAD 1和HAD 2/LAD 2大鼠系的近交。 除了生产P/NP和HAD/LAD外， 动物生产中心还将维持一个HARF/LARF群体， （来自多伦多的酒精偏好/非偏好大鼠品系，基于 限制饮用水范例）大鼠，以便可以提高F2， 数量性状基因座分析。 由于我们最近已经描述了在细胞中的分子异常， 在NP大鼠中观察到的血栓病，现在已经发展为近交系P和 NP大鼠品系（兄弟×姐妹交配22代），动物 生产核心现在可以启动项目， 缺乏出血性疾病的同类NP系。 此外，本发明还 将进行实验以确定P和HAD大鼠是否会 发展成严重的肝病（即，肝纤维化伴或不伴 肝硬化）通过自愿和/或强迫乙醇饮用和 有利于酒精诱导的肝损伤和纤维化的条件。

The Animal Production Core will continue to supply selectively bred and inbred P and NP (alcohol-preferring and -non preferring) rats as well as selectively bred HAD and LAD (high and low alcohol-drinking) rats for experimental use. These rodents will be made available to researchers supported by or affiliated with the ARC at Indiana University, and to collaborating, and independent researchers at other Institutions. In order to accomplish this objective: 1) P and NP lines of rats will continue to be selectively bred by a relaxed selection procedure. 2) P and NP inbred strains, four each, will be maintained by continuing inbreeding. 3) The second and third litters of the selectively bred HAD1/LAD1 and HAD2/LAD2 rat lines from each generation will be produced for research use. 4) Inbreeding of HAD1/LAD1 and HAD2/LAD2 rat lines will be continued. In addition to producing P/NP and HAD/LAD selectively bred and inbred rats, the Animal Production Core will also maintain a colony of HARF/LARF (alcohol-preferring/non preferring rat lines from Toronto based on a limited-access drinking paradigm) rats so that F2s can be raised for Quantitative Trait Loci analysis. Since we recently have characterized the molecular abnormality in the thrombopathia that is seen in NP rats and now have developed inbred P and NP rat strains (brother x sister mating for 22 generations), the Animal Production Core is now in the position to initiate the project to create a congenic NP line that lacks the bleeding disorder. Additionally, experiments will be carried out to determine whether P and HAD rats will develop serious liver disease (i.e., hepatic fibrosis with or without cirrhosis) through voluntary and/or forced ethanol drinking and under conditions that favor alcohol-induced hepatic injury and fibrogenesis.