CORE--ANIMAL PRODUCTION

核心——畜牧生产

• 批准号: 6352520
• 负责人: TING-KAI K LI
• 金额: $ 21.25万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-21 至 2000-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6352520
• 关键词: alcoholic beverage consumption; alcoholic liver cirrhosis; animal breeding; animal colony; behavior test; biological models; biomedical facility; genetic models; genetic strain; inbreeding; laboratory rat; linkage mapping; preference

The Animal Production Core will continue to supply selectively bred and inbred P and NP (alcohol-preferring and -non preferring) rats as well as selectively bred HAD and LAD (high and low alcohol-drinking) rats for experimental use. These rodents will be made available to researchers supported by or affiliated with the ARC at Indiana University, and to collaborating, and independent researchers at other Institutions. In order to accomplish this objective: 1) P and NP lines of rats will continue to be selectively bred by a relaxed selection procedure. 2) P and NP inbred strains, four each, will be maintained by continuing inbreeding. 3) The second and third litters of the selectively bred HAD1/LAD1 and HAD2/LAD2 rat lines from each generation will be produced for research use. 4) Inbreeding of HAD1/LAD1 and HAD2/LAD2 rat lines will be continued. In addition to producing P/NP and HAD/LAD selectively bred and inbred rats, the Animal Production Core will also maintain a colony of HARF/LARF (alcohol-preferring/non preferring rat lines from Toronto based on a limited-access drinking paradigm) rats so that F2s can be raised for Quantitative Trait Loci analysis. Since we recently have characterized the molecular abnormality in the thrombopathia that is seen in NP rats and now have developed inbred P and NP rat strains (brother x sister mating for 22 generations), the Animal Production Core is now in the position to initiate the project to create a congenic NP line that lacks the bleeding disorder. Additionally, experiments will be carried out to determine whether P and HAD rats will develop serious liver disease (i.e., hepatic fibrosis with or without cirrhosis) through voluntary and/or forced ethanol drinking and under conditions that favor alcohol-induced hepatic injury and fibrogenesis.

畜牧生产核心将继续提供有选择的养殖 以及近交系P和NP(偏爱酒精和不偏爱酒精)大鼠 作为选择性饲养的HAD和LAD(高酒精和低酒精)大鼠 试验性使用。这些啮齿动物将提供给研究人员 由印第安纳大学ARC支持或附属于ARC，并 合作，以及其他机构的独立研究人员。按顺序 为实现这一目标：1)大鼠的P和NP系将继续 通过宽松的选择程序有选择地繁殖。2)P、NP自交系 品系，每个四个，将通过持续的近亲繁殖来维持。3) 选育的HAD1/LAD1和HAD2/LAD2的第二窝和第三窝 每一代的老鼠品系都将被用于研究。4) 将继续进行HAD1/LAD1和HAD2/LAD2大鼠品系的近亲交配。 除了生产P/NP和HAD/LAD选择性繁殖和近交系 大鼠，动物生产核心也将维持一个HARF/LIFF群体 (酒精偏好/非偏好基于多伦多的老鼠品系 限制饮酒范例)大鼠，以便F2可以被饲养 数量性状基因座分析。 因为我们最近已经描述了分子异常在 出现在NP大鼠身上的血栓病，现在已经发展为近交系P和 NP大鼠品系(兄妹交配22代)，动物 Production Core现在可以启动要创建的项目 一种没有出血障碍的同源NP系。另外， 将进行实验以确定P和HAD大鼠是否会 发展成严重的肝病(即，肝纤维化伴或不伴 肝硬变)通过自愿和/或强迫饮酒及以下 有利于酒精性肝损伤和肝纤维化形成的条件。