Tomographic Microscope for Assessing Microcirculation

用于评估微循环的断层扫描显微镜

• 批准号: 6961471
• 负责人: PAUL SHNITSER
• 金额: $ 3.11万
• 依托单位: PHYSICAL OPTICS CORPORATION
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-20 至 2005-03-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6961471
• 关键词: arterioles; bioimaging /biomedical imaging; biomedical equipment development; chick embryo; computer program /software; erythrocytes; imaging /visualization /scanning; microcirculation; microscopy; noninvasive diagnosis; optical tomography; optics; portable biomedical equipment

DESCRIPTION (provided by applicant): Many widespread diseases are caused by or manifested in abnormalities in blood microcirculation. There is a pressing need for a noninvasive device to collect maximum information about the structure of the microcirculation network, the functional parameters of blood microcirculation such as a blood flow velocity and blood oxygen saturation, and tissue morphology. To satisfy this need, Physical Optics Corporation (POC) proposes to develop a new multiwavelength optical coherent Doppler tomography (MOCDOT) microscope capable of 3D visualization of tissue morphology to a depth up to several millimeters, and of separate visualization of the microvascular network with measurement of the blood flow velocities in each component of this network and of arteriolar blood oxygenation. These multiple functions will be achieved by the unique combination of en-face optical coherence tomography with heterodyne measurements of the Doppler shift induced by light scattering by moving red blood cells. In Phase I, POC will fabricate a prototype of the MOCDOT microscope, and will demonstrate the performance on a chick embryo. After evaluating the test results, POC will optimize the MOCDOT design in preparation for developing a Phase II working prototype for clinical testing on patients who might have diabetic microangiopathy.

描述（申请人提供）：许多广泛传播的疾病是由血液微循环异常引起或表现为血液微循环异常。迫切需要一种无创设备来最大限度地收集微循环网络结构、血流速度、血氧饱和度等血液微循环功能参数以及组织形态等信息。为了满足这一需求，物理光学公司 (POC) 提议开发一种新型多波长光学相干多普勒断层扫描 (MOCDOT) 显微镜，能够对深度达几毫米的组织形态进行 3D 可视化，并通过测量该网络每个组件的血流速度和小动脉血液氧合来单独可视化微血管网络。这些多种功能将通过正面光学相干断层扫描与移动红细胞光散射引起的多普勒频移的外差测量的独特组合来实现。在第一阶段，POC 将制造 MOCDOT 显微镜的原型，并将在鸡胚胎上展示其性能。在评估测试结果后，POC将优化MOCDOT设计，准备开发II期工作原型，用于对可能患有糖尿病微血管病变的患者进行临床测试。