MICROBIAL PATHOGENS IN RODENT GAMETE AND EMBRYO BANKING

啮齿动物配子和胚胎库中的微生物病原体

• 批准号: 6937711
• 负责人: LELA K. RILEY
• 金额: $ 25.23万
• 依托单位: UNIVERSITY OF MISSOURI-COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-30 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6937711
• 关键词: Rodentias; antiinfective agents; cryopreservation; cryoprotective agents; disease /disorder model; embryo /fetus preservation; environmental contamination; germ cells; laboratory mouse; laboratory rat; method development; microorganism classification; microorganism disease chemotherapy; microorganism growth; polymerase chain reaction; tissue resource /registry; vertical transmission; virulence

DESCRIPTION (Provided by applicant): With the pending completion of the Human Genome Project, scientists will soon know the sequence of all human genes. This knowledge will create an unprecedented need for new rodent models to identify and understand gene function to test new therapeutic strategies aimed at alleviating human diseases. Maintenance of the exponentially increasing numbers of these mutant strains as live animals will not be feasible. Cryopreservation and genome banking of spermatozoa and oocytes (gametes) and embryos will be mandatory. However, there are two major impediments to genome banking of rodent models. Many mutant mouse and rat lines are infected with microbial pathogens. An understanding of the risks of transmission of infectious agents through gametes and embryos must be developed. Preservation of gametes and embryos will be compromised if they are contaminated with agents that lead to infection in the regenerated mutant lines and cause outbreaks of infectious disease. Methods to efficiently freeze mouse embryos and regenerate strains stocks from frozen stocks via embryo transfer are well established for the mouse, but little work has been done in developing efficient rat embryo cryopreservation methodology. The Specific Aims of this application are to: 1) determine which of the commonly found murine pathogens contaminate mouse and rat gametes and embryos; 2) develop methods to remove microbial pathogens from rodent gametes and embryos and thus, decrease the likelihood of their transmission during genome banking; and 3) develop improved rat embryo cryopreservation methods.

描述（申请人提供）：随着人类基因组计划的即将完成，科学家们将很快知道所有人类基因的序列。 这些知识将创造一个前所未有的需要新的啮齿动物模型， 识别和了解基因功能，以测试新的治疗策略， 减轻人类疾病。维持指数增长 将这些突变株作为活动物的数量是不可行的。 精子和卵母细胞（配子）的冷冻保存和基因组建库， 胚胎是强制性的。 然而，啮齿动物模型的基因组库存在两个主要障碍。 许多突变的小鼠和大鼠品系被微生物病原体感染。一个 了解传染源通过以下途径传播的风险 必须发育配子和胚胎。配子和胚胎的保存 如果它们被某种物质污染， 在再生突变株系中感染并引起传染性疾病的爆发 疾病高效冷冻小鼠胚胎和再生品系的方法 通过胚胎移植从冷冻原种中获得的原种已经很好地建立， 小鼠，但在开发有效的大鼠胚胎方面的工作很少 冷冻保存方法学。本申请的具体目的是： 1)确定哪些常见的鼠病原体污染小鼠 以及大鼠配子和胚胎; 2）开发去除微生物病原体的方法 从啮齿动物的配子和胚胎，从而减少他们的可能性， 在基因组建库期间传播;以及3）开发改良的大鼠胚胎 冷冻保存方法