Diet/Intramyocellular Lipid/Endurance Performance

饮食/肌细胞内脂质/耐力表现

• 批准号: 6845327
• 负责人: D ENETTE LARSON-MEYER
• 金额: $ 9.66万
• 依托单位: UNIVERSITY OF WYOMING
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-01 至 2006-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6845327
• 关键词: adipocytes; bioenergetics; biopsy; body composition; carbohydrate metabolism; cell morphology; clinical research; computed axial tomography; diet; dietary lipid; electron microscopy; exercise; fatty acid metabolism; fatty acid transport; female; human subject; lipid metabolism; muscle cells; muscle metabolism; muscle strength; noninsulin dependent diabetes mellitus; nuclear magnetic resonance spectroscopy; nutrition related tag; photon absorptiometry; statistics /biometry; striated muscles

DESCRIPTION (provided by applicant): For this Career Development Award application, I will capitalize and build on my past training experience which includes: a) a Master's Degree in Clinical Dietetics; b) experience as a Research Dietitian at the NIDDK in Phoenix, during which I investigated the relationship between food intake and energy metabolism; c) a Doctorate Degree from the University of Alabama at Birmingham, where my research focused on the use of 31p Magnetic Resonance (MR) Spectopscopy to measure skeletal muscle metabolism; and d) a Post-doctoral Fellowship at UAB where I used 1H MR Spectroscopic Imaging to look at the impact of dietary fat on intramyocellular lipid content. As part of my overall project "Impact of Diet on Intramyocellular Lipid, Endurance Performance and Insulin Sensitivity", I am proposing to learn research skills that will help complement my current knowledge/capabilities. These skills include the technologies for measuring: a) skeletal muscle lipid and glycogen content by biochemical and histochemical techniques; b) muscle fat infiltration by computed tomography; c) intracellular lipid content in liver using 1H MR spectroscopy; d) carbohydrate metabolism using 13C- MR spectroscopy; e) insulin sensitivity by the hyperinsulinemic euglycemic clamp; and f) lipid metabolism using stable isotope infusion. I will also increase my knowledge of basic exercise physiology techniques, clinical research administration and bioethics. My project involves three individual studies (projects) that have been peer reviewed and funded. Project 1 will address whether differences in the amount of fat stored within the skeletal muscle as a result of endurance exercise and either a very-low fat or moderate-fat diet will result in differences in endurance performance. Project 2 will focus on the influence of either a low-fat or a moderate fat diet on adaptation to 16-weeks of exercise training. Project 3 will test whether lipid content in skeletal muscle and liver correlates positively with abdominal subcutaneous adipocyte size in type 2 diabetics and obese non-diabetics, and negatively with insulin sensitivity. My involvement in these projects will provide the perfect opportunity to develop these research skills. To achieve my career development goals, I will be mentored by: Drs Eric Ravussin (Sponsor), Claude Bouchard, Donna H. Ryan, David E. Kelley, Gerald I. Shulman and Mark A.Tarnopolsky (consulting sponsors). Completion of this mentored training will position me to achieve my long-term career objective of becoming an independent investigator with expertise in energy and macronutrient metabolism. There is ample equipment and facilities at the Pennington Center to support the projects and career development plan proposed.

描述（由申请人提供）： 对于这个职业发展奖的申请，我将利用和建立在我过去的培训经验，其中包括：a）临床营养学硕士学位; B）在凤凰城的NIDDK担任研究营养师的经验，在此期间，我调查了食物摄入量和能量代谢之间的关系; c）伯明翰亚拉巴马大学的博士学位，我的研究集中在使用31 p磁共振（MR）光谱仪测量骨骼肌代谢;和d）在UAB的博士后奖学金，我使用1H MR光谱成像来观察膳食脂肪对肌细胞内脂质含量的影响。作为我的整体项目“饮食对肌内脂质，耐力表现和胰岛素敏感性的影响”的一部分，我建议学习研究技能，这将有助于补充我目前的知识/能力。这些技能包括测量技术：a）通过生物化学和组织化学技术测量骨骼肌脂质和糖原含量; B）通过计算机断层扫描测量肌肉脂肪浸润; c）使用1H MR光谱法测量肝脏中的细胞内脂质含量; d）使用13 C-MR光谱法测量碳水化合物代谢; e）通过高胰岛素血正葡萄糖钳夹测量胰岛素敏感性;以及f）使用稳定同位素输注测量脂质代谢。我还将增加我的基本运动生理学技术，临床研究管理和生物伦理学的知识。我的项目涉及三个单独的研究（项目），这些研究已经过同行评审和资助。项目1将讨论耐力运动和极低脂肪或中等脂肪饮食导致的骨骼肌内储存的脂肪量差异是否会导致耐力表现的差异。项目2将重点关注低脂肪或中等脂肪饮食对适应16周运动训练的影响。项目3将测试骨骼肌和肝脏中的脂质含量是否与2型糖尿病患者和肥胖非糖尿病患者的腹部皮下脂肪细胞大小呈正相关，与胰岛素敏感性呈负相关。我在这些项目的参与将提供一个完美的机会来发展这些研究技能。为了实现我的职业发展目标，我将接受以下导师的指导：Eric Ravussin博士（赞助商），Claude Bouchard，Donna H。作者：大卫E. Kelley，Gerald I. Shulman和Mark A.Tarnopolsky（咨询赞助商）。完成这一指导培训将使我能够实现我的长期职业目标，成为一名在能量和大量营养素代谢方面具有专业知识的独立调查员。彭宁顿中心有充足的设备和设施来支持所提出的项目和职业发展计划。

项目成果

Effects of endurance running and dietary fat on circulating ghrelin and peptide YY.

耐力跑和膳食脂肪对循环胃饥饿素和肽 YY 的影响。

• 发表时间: 2009
• 期刊: Journal of sports science & medicine
• 影响因子: 3.2
• 作者: Russel,RyanR;Willis,KentzS;Ravussin,Eric;Larson-Meyer,EnetteD
• 通讯作者: Larson-Meyer,EnetteD

Effect of dietary fat on serum and intramyocellular lipids and running performance.

膳食脂肪对血清和肌细胞内脂质以及跑步性能的影响。

• DOI: 10.1249/mss.0b013e318164cb33
• 发表时间: 2008
• 期刊: Medicine and science in sports and exercise
• 影响因子: 4.1
• 作者: Larson-Meyer,DEnette;Borkhsenious,OlgaN;Gullett,JonC;Russell,RyanR;Devries,MichaelaC;Smith,StevenR;Ravussin,Eric
• 通讯作者: Ravussin,Eric

Vitamin D status and biomarkers of inflammation in runners.

• DOI: 10.2147/oajsm.s31022
• 发表时间: 2012
• 期刊: Open access journal of sports medicine
• 影响因子: 2.4
• 作者: Willis KS;Smith DT;Broughton KS;Larson-Meyer DE
• 通讯作者: Larson-Meyer DE