CORE--LAB ANIMAL HEALTH

核心--实验动物健康

• 批准号: 7141894
• 负责人: Robert C Young
• 金额: $ 12.42万
• 依托单位: FOX CHASE CANCER CENTER
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-10 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7141894
• 关键词: Rodentias; animal colony; biomedical facility; germ free condition; neoplasm /cancer; veterinary medicine

The Laboratory Animal Health Facility (LAHF) supports an ever-increasing demand for the use of animals in the Fox Chase Cancer Center's (FCCC) Laboratory Animal Facility (LAF). In 2003, the LAHF supported 46 investigators in all 11 research Programs from all three Divisions at the Center. The vast majority (96%) of use was for peer-reviewed, funded research. The increase in mouse usage during the past five years was 116%, and the use of rodents is anticipated to expand approximately 100% in the next five years. Even as importation of mice from collaborators has increased by over 50% in the past five years, the Facility has maintained and improved the health of the colony. All new strains imported from other Facilities are held in quarantine and monitored for murine disease by serology and polymerase chain reaction (PCR) techniques; most have to be rederived by germ-free cesarean or embryo transfer and raised on disease-free foster mothers in microisolator units before permitted into the main research Facility. Over 81 such cesarean rederivations were done in 2003. The health of animals and their freedom from murine pathogens is constantly monitored by sentinel animals in each animal room, and the entire research Facility has remained viral antibody-free since 1998, Severe immune-deficient mice are bred and successfully live up to two years in the FCCC research colony. Pneumocystis, Pasteurella and Helicobacter have already been eliminated from all breeding and immunodeficient rodent areas, and continued systematic rederivation combined with increased microisolator housing is expected to completely eliminate these agents from all FCCC colonies within two years. In 2003, the LAHF performed 11,967 tests (5,600 microbiological cultures, 3,179 ELISA assays, 2,088 (PCR) assays, and 1,100 necropsies and the number of service hours of direct support to research investigators has increased 210% (1,912 in 2003). Technical support includes animal surgery, antibody production in rabbits, mice and rats, collecting samples from animals, tumor line maintenance, necropsies, tumor and cell culture screening, and microbiological/serological testing. The need for technical services has increased almost four-fold from 1998 (616 service hours) to 2003 (1,912 service hours). This increase is expected to continue parallel to the increase in animal numbers, and an increase of one FTE is projected.

实验室动物卫生设施（LAHF）支持对使用 Fox Chase Cancer Center（FCCC）Laboratory Animal Facility（LAF）中的动物。2003年， LAHF支持了46名研究人员，他们来自美国国家航空航天局所有三个部门的所有11个研究项目， 中心绝大多数（96%）用于同行评审，资助研究。在过去五年中，老鼠的使用增加了116%，预计啮齿动物的使用将 在未来五年内扩大约100%。尽管过去五年从合作者进口的小鼠增加了50%以上，但该设施仍维持并改善了殖民地的健康状况。从其他机构进口的所有新菌株均进行隔离，并通过血清学和聚合酶链反应（PCR）技术监测鼠疾病;在允许进入主要研究机构之前，大多数必须通过无菌剖腹产或胚胎移植进行再衍生，并在微隔离器单元中在无病养母身上饲养。在2003年进行了超过81例这样的剖宫产手术。动物的健康和他们的自由从鼠病原体是不断监测哨兵动物在每个动物房，整个研究设施一直保持无病毒抗体自1998年以来，严重的免疫缺陷小鼠繁殖，并成功地生活在FCCC研究殖民地长达两年。肺孢子虫、巴氏杆菌和螺杆菌已经从所有繁殖和免疫缺陷啮齿动物区域中消除，持续的系统性再衍生结合增加的微隔离器住房预计将在两年内从所有FCCC菌落中完全消除这些病原体。2003年，LAHF进行了11，967次检测（5，600次微生物培养、3，179次ELISA检测、2，088次PCR检测和1，100次尸检），直接支持研究人员的服务小时数增加了210%（2003年为1，912小时）。技术支持包括动物外科手术、兔、小鼠和大鼠抗体生产、动物样本采集、肿瘤系维持、尸检、肿瘤和细胞培养筛选以及微生物/血清学检测。从1998年（616个服务小时）到2003年（1 912个服务小时），对技术服务的需求几乎增加了四倍。预计这一增加将继续与动物数量的增加平行，预计将增加一个FTE。