CORE--LAB ANIMAL HEALTH

实验动物卫生设施(LAHF)支持不断增长的使用需求 福克斯·蔡斯癌症中心的实验动物设施(LAF)中的动物。二零零三年， LAHF支持了来自所有三个部门的所有11个研究项目的46名研究人员 中心。绝大多数(96%)用于同行评议、资助的研究。在过去的五年中，老鼠的使用增加了116%，啮齿动物的使用预计将 在未来五年内实现约100%的扩张。尽管从合作者那里进口的小鼠在过去五年中增加了50%以上，但该设施保持并改善了殖民地的健康。从其他设施进口的所有新菌株都被隔离，并通过血清学和聚合酶链式反应(PCR)技术监测老鼠疾病；大多数必须通过无菌剖腹产或胚胎移植重新衍生，并在微型隔离单元中由无病寄养母亲饲养，然后才允许进入主要研究设施。2003年，超过81例这样的剖腹产手术完成。动物的健康及其免受鼠类病原体的影响由每个动物房间的哨兵动物持续监测，整个研究机构自1998年以来一直保持无病毒抗体，自1998年以来，在fccc研究群体中饲养了严重免疫缺陷小鼠，并成功存活长达两年。肺孢子虫、巴氏杆菌和幽门螺杆菌已经从所有繁殖和免疫缺陷啮齿类动物地区消除，继续系统的重新派生和增加微隔离器外壳有望在两年内从所有fccc菌落中完全消除这些病原体。2003年，研究所进行了11,967次检测(5,600次微生物培养、3,179次酶联免疫吸附试验、2,088次(聚合酶链式反应)检测和1,100例尸检)，直接支持研究人员的服务小时数增加了210%(2003年为1,912小时)。技术支持包括动物手术、兔、小鼠和大鼠的抗体生产、动物样本采集、肿瘤系维护、身体解剖、肿瘤和细胞培养筛选以及微生物学/血清学测试。从1998年(616个服务小时)到2003年(1912个服务小时)，对技术服务的需求几乎增加了四倍。这一增长预计将继续与动物数量的增加并行，预计将增加1 FTE。

The Laboratory Animal Health Facility (LAHF) supports an ever-increasing demand for the use of animals in the Fox Chase Cancer Center's (FCCC) Laboratory Animal Facility (LAF). In 2003, the LAHF supported 46 investigators in all 11 research Programs from all three Divisions at the Center. The vast majority (96%) of use was for peer-reviewed, funded research. The increase in mouse usage during the past five years was 116%, and the use of rodents is anticipated to expand approximately 100% in the next five years. Even as importation of mice from collaborators has increased by over 50% in the past five years, the Facility has maintained and improved the health of the colony. All new strains imported from other Facilities are held in quarantine and monitored for murine disease by serology and polymerase chain reaction (PCR) techniques; most have to be rederived by germ-free cesarean or embryo transfer and raised on disease-free foster mothers in microisolator units before permitted into the main research Facility. Over 81 such cesarean rederivations were done in 2003. The health of animals and their freedom from murine pathogens is constantly monitored by sentinel animals in each animal room, and the entire research Facility has remained viral antibody-free since 1998, Severe immune-deficient mice are bred and successfully live up to two years in the FCCC research colony. Pneumocystis, Pasteurella and Helicobacter have already been eliminated from all breeding and immunodeficient rodent areas, and continued systematic rederivation combined with increased microisolator housing is expected to completely eliminate these agents from all FCCC colonies within two years. In 2003, the LAHF performed 11,967 tests (5,600 microbiological cultures, 3,179 ELISA assays, 2,088 (PCR) assays, and 1,100 necropsies and the number of service hours of direct support to research investigators has increased 210% (1,912 in 2003). Technical support includes animal surgery, antibody production in rabbits, mice and rats, collecting samples from animals, tumor line maintenance, necropsies, tumor and cell culture screening, and microbiological/serological testing. The need for technical services has increased almost four-fold from 1998 (616 service hours) to 2003 (1,912 service hours). This increase is expected to continue parallel to the increase in animal numbers, and an increase of one FTE is projected.