CORE--DNA MICROARRAY FACILITY

核心——DNA微阵列设备

• 批准号: 7142491
• 负责人: Robert C Young
• 金额: $ 11.82万
• 依托单位: FOX CHASE CANCER CENTER
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-10 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7142491
• 关键词: DNA; biomedical facility; microarray technology; neoplasm /cancer

Microarray technology has broad applications in cancer research such as identification of cancer markers and gene expression profiling for diagnosis, treatment, and prognosis. Maintenance of a Core DNA Microarray Facility in a comprehensive cancer research center is critical for the success of a growing number of investigators with peer-reviewed funding. The Microarray Facility was established in 1999 using institutional and developmental funds from the CCSG. The Facility provides both printed cDNA and oligo arrays, for investigators to perform gene expression analysis, and full microarray services from RNA sample labeling to array scanning, for those investigators who do not have the capacity to perform microarray experiments in their own laboratories. The Facility also helps investigators to design and print custom microarrays to meet their specific research goals. The Facility is used by 20 investigators, all with peer-reviewed funding, from 9 research Programs in all three Divisions of Fox Chase Cancer Center (FCCC). The microarray usage has increased more than 200% from the year 2002 to 2003 and reached 1,300 microarrays per year. With an increasing number of research grants involving microarray analysis awarded recently, and the establishment of a Laser Capture Microdissection Facility at the Center, combined with RNA amplification procedure which enables small clinical cancer samples to be applied to microarray analysis, we anticipate a 100%-200% increase over the next five years. The microarray products of the Facility include human 40k cDNA and 30k oligo arrays, mouse 15k cDNA and 32k oligo arrays, and 6k yeast oligo arrays. A rat oligo library will be acquired to meet the needs of those investigators using rat models to study breast and ovarian oncogenesis. The Facility has also designed and produced customer design specific arrays, such as DNA repair process-related gene arrays. Standard microarray procedures and optimized labeling and hybridization kits, as well as a Microarray Training Program, have been made available to microarray users. A comprehensive microarray quality control procedure has been implemented to ensure the quality of microarray products and services. The Facility coordinates with the Bioinformatics Facility for microarray data analysis and management.

微阵列技术在癌症研究中具有广泛的应用，例如鉴定 用于诊断、治疗和预后的癌症标志物和基因表达谱。 一个综合性癌症研究中心核心DNA微阵列设备的维护， 这对于越来越多的研究人员获得同行评审资金的成功至关重要。的 微阵列设施于1999年建立，使用来自 CCSG。该设施提供打印的cDNA和oligo阵列，供研究人员进行基因表达分析，并为那些没有能力在自己的实验室进行微阵列实验的研究人员提供从RNA样品标记到阵列扫描的完整微阵列服务。该设施还帮助研究人员设计和打印定制的微阵列，以满足他们的特定研究目标。该设施由20名研究人员使用，所有研究人员都有同行评审的资金，来自福克斯蔡斯癌症中心（FCCC）所有三个部门的9个研究项目。从2002年到2003年，微阵列的使用增加了200%以上，每年达到1,300个微阵列。随着最近越来越多的研究赠款涉及微阵列分析，并在中心建立了激光捕获显微切割设备，结合RNA扩增程序，使小的临床癌症样品能够应用于微阵列分析，我们预计在未来五年内将增加100%-200%。该设施的微阵列产品包括人40 k cDNA和30 k oligo阵列，小鼠15 k cDNA和32 k oligo阵列，以及6 k酵母oligo阵列。将获得大鼠寡核苷酸文库，以满足使用大鼠模型研究乳腺和卵巢肿瘤发生的研究者的需求。该设施还设计和生产客户设计的特定阵列，如DNA修复过程相关的基因阵列。标准的微阵列程序和优化的标记和杂交试剂盒，以及微阵列培训计划，已提供给微阵列用户。一个全面的微阵列质量控制程序已经实施，以确保微阵列产品和服务的质量。该设施与生物信息学设施协调，进行微阵列数据分析和管理。