CORE--CELL SORTING

核心——细胞分选

基本信息

  • 批准号:
    7142508
  • 负责人:
  • 金额:
    $ 7.36万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2005
  • 资助国家:
    美国
  • 起止时间:
    2005-09-10 至 2010-06-30
  • 项目状态:
    已结题

项目摘要

The Research Cell Sorting and Flow Cytometry Facility offers Fox Chase Cancer Center (FCCC) investigators the capability of analyzing cells by up to 14 independent measurements, 12 of which are based on immunofluorescence and two on light scattering. This Facility is used by 36 peer-reviewed funded investigators (an increase from 21 or 71% from the previous review) across 9 Research Programs and by investigators within all three FCCC Divisions. Ninety-five percent (95%) of this usage is by peer-reviewed funded investigators. Major applications of the flow cytometer include discrimination and isolation (sorting) of cell subpopulations (at up to 20,000 cells/sec), isolation of rare variant (1/10[7]) cell lines, characterization of tumors or cell lines, and single cell cloning of cell lines by direct deposition into 96-well plates. In addition to providing many more fluorescence detectors (increase from 5 to 12), the recently upgraded sorter provides high-speed 4-stream sorting and digital detection. A UV-capable laser enables measurement of calcium flux and viable cell DNA content. In addition to the 3-laser 12-color cell sorter (FACS-VantageSE/DiVa), the Facility also includes an analysis-only 8-color LSR-II and a three-color FACScan. The Facility aids users with advice on experimental design (including availability of reagents and appropriate fluorochromes), sample preparation, and data interpretation. Software developed with the FCCC Bioinformatics group, the Flow-LIMS, is used for data annotation, organization, and archiving. Analysis is done on investigator's laboratory computers using a highly interactive, graphical software program, FlowJo. Hour usage (currently estimated at over 80% of theoretical capacity) has increased 65% since the last review and is projected to increase similarly over the next five years. Equitable access to the Facility is assured by a Faculty Oversight Committee.
研究细胞分选和流式细胞术设备提供福克斯蔡斯癌症中心

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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Robert C Young其他文献

Anticholinergic effects and plasma desipramine levels
抗胆碱能作用和血浆地昔帕明水平
Age and Serum Fluphenazine Concentrations
年龄与氟奋乃静血清浓度
  • DOI:
    10.1038/sj.npp.1380230
  • 发表时间:
    2003-07-25
  • 期刊:
  • 影响因子:
    7.100
  • 作者:
    James J Weisbard;Robert C Young
  • 通讯作者:
    Robert C Young

Robert C Young的其他文献

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{{ truncateString('Robert C Young', 18)}}的其他基金

CORE--RESEARCH CYTOGENETICS
核心--研究细胞遗传学
  • 批准号:
    7142523
  • 财政年份:
    2005
  • 资助金额:
    $ 7.36万
  • 项目类别:
CORE--LAB ANIMAL HEALTH
核心--实验动物健康
  • 批准号:
    7141894
  • 财政年份:
    2005
  • 资助金额:
    $ 7.36万
  • 项目类别:
CORE--HISTOPATHOLOGY
核心--组织病理学
  • 批准号:
    7142512
  • 财政年份:
    2005
  • 资助金额:
    $ 7.36万
  • 项目类别:
CORE--GENETIC MONITORING
核心——基因监测
  • 批准号:
    7141890
  • 财政年份:
    2005
  • 资助金额:
    $ 7.36万
  • 项目类别:
CORE--TUMOR BANK
核心——肿瘤库
  • 批准号:
    7142525
  • 财政年份:
    2005
  • 资助金额:
    $ 7.36万
  • 项目类别:
CORE--DNA MICROARRAY FACILITY
核心——DNA微阵列设备
  • 批准号:
    7142491
  • 财政年份:
    2005
  • 资助金额:
    $ 7.36万
  • 项目类别:
CORE--MOLECULAR MODELING FACILITY
核心——分子模拟设施
  • 批准号:
    7142498
  • 财政年份:
    2005
  • 资助金额:
    $ 7.36万
  • 项目类别:
CORE--HYBRIDOMA
核心--杂交瘤
  • 批准号:
    7142516
  • 财政年份:
    2005
  • 资助金额:
    $ 7.36万
  • 项目类别:
CORE--CELL CULTURE
核心--细胞培养
  • 批准号:
    7142502
  • 财政年份:
    2005
  • 资助金额:
    $ 7.36万
  • 项目类别:
CORE--BIOSAMPLE REPOSITORY
核心--BIOSAMPLE存储库
  • 批准号:
    7142518
  • 财政年份:
    2005
  • 资助金额:
    $ 7.36万
  • 项目类别:

相似海外基金

ShEEP Request for a Replacement Cell Sorting Flow Cytometer for the Flow Cytometry Core Facility
ShEEP 请求为流式细胞术核心设施更换细胞分选流式细胞仪
  • 批准号:
    9793614
  • 财政年份:
    2019
  • 资助金额:
    $ 7.36万
  • 项目类别:
Flow Cytometry Cell Sorting Analysis Research Platform.
流式细胞术细胞分选分析研究平台。
  • 批准号:
    472604-2015
  • 财政年份:
    2014
  • 资助金额:
    $ 7.36万
  • 项目类别:
    Research Tools and Instruments - Category 1 (<$150,000)
Flow Cytometry and Cell Sorting Shared Resource Facility
流式细胞术和细胞分选共享资源设施
  • 批准号:
    10470107
  • 财政年份:
    2013
  • 资助金额:
    $ 7.36万
  • 项目类别:
FLOW CYTOMETRY AND CELL SORTING
流式细胞术和细胞分选
  • 批准号:
    8436444
  • 财政年份:
    2013
  • 资助金额:
    $ 7.36万
  • 项目类别:
Flow Cytometry and Cell Sorting Shared Resource Facility
流式细胞术和细胞分选共享资源设施
  • 批准号:
    10204888
  • 财政年份:
    2013
  • 资助金额:
    $ 7.36万
  • 项目类别:
Flow Cytometry and Cell Sorting
流式细胞术和细胞分选
  • 批准号:
    8738007
  • 财政年份:
    2012
  • 资助金额:
    $ 7.36万
  • 项目类别:
FLOW CYTOMETRY ANALYSIS/ HIGH SPEED CELL SORTING
流式细胞术分析/高速细胞分选
  • 批准号:
    8360463
  • 财政年份:
    2011
  • 资助金额:
    $ 7.36万
  • 项目类别:
MRI: Acquisition of Mobile Flow Cytometry and Cell Sorting Facility for Microbiological Research and Minority Student Training
MRI:购置移动流式细胞术和细胞分选设备,用于微生物学研究和少数民族学生培训
  • 批准号:
    1126749
  • 财政年份:
    2011
  • 资助金额:
    $ 7.36万
  • 项目类别:
    Standard Grant
FLOW CYTOMETRY AND CELL SORTING
流式细胞术和细胞分选
  • 批准号:
    8364982
  • 财政年份:
    2011
  • 资助金额:
    $ 7.36万
  • 项目类别:
Flow Cytometry/Cell Sorting
流式细胞术/细胞分选
  • 批准号:
    8180852
  • 财政年份:
    2010
  • 资助金额:
    $ 7.36万
  • 项目类别:
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