Molecular Definition Of Filarial & Nonfilarial Genes

利用EST（表达序列标签）方法，基于1687个EST序列，我们鉴定出了965个以上的班氏乌氏菌微丝虫基因簇。有趣的是，这些聚集的ESTs中有惊人的高比例（584/965;60%）含有假定的信号肽，只有6个似乎是GPI锚定的。与马来Brugia malayi (Bm) mf ESTs/集群相比，45%的Wb集群与Bm ESTs匹配（一致性范围为86-100%），而较少（22%）与盘尾丝虫（Ov） mf ESTs匹配（一致性范围为80-100%）。

Using an EST (expressed sequence tag) approach, we have been able to identify greater than 965 gene clusters from Wuchereria bancrofti microfilariae based on1687 ESTs. Interestingly, a surprisingly high percentage (584/965; 60%) of these clustered ESTs contain a putative signal peptide, and only 6 appear to be GPI anchored. When compared to Brugia malayi (Bm) mf ESTs/Clusters, 45% of the Wb clusters had matches to Bm ESTs (identities ranging from 86-100%) whereas fewer (22%) matched Onchocerca volvulus (Ov) mf ESTs (with identities ranging from 80-100%). A similar approach has been taken with Strongyloides stercoralis with >10000 ESTs being done from two separate lifecylcle stages. Comparisons between this parasitic nematode and the free-living C. elegans has been perfomed; the data demonstrate the vast differences in gene expresssion between parasitic and non-parasitic nematodes (Mitreva, DM, McCarter, JP. Marin, J, Dante, M, Wylie, T, Chipelli, B, Pape, D, Clifton, SW. Nutman, TB, Waterston, RH. Comparative Genomics of Gene Expression in the Parasitic and Free-living Nematodes Strongyloides stercoralis and Caenorhabditis elegans. Genome Res 14: 209-220, 2004).