CLINICAL AND THERAPEUTIC STUDIES OF HUMAN FILARIASIS

人类丝虫病的临床和治疗研究

• 批准号: 6431567
• 负责人: THOMAS B NUTMAN
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6431567
• 关键词: anthelmintics; clinical research; clinical trials; disease /disorder classification; enzyme linked immunosorbent assay; epidemiology; filariasis; human subject; human therapy evaluation; immunity; onchocerciasis; parasitic disease chemotherapy; parasitic disease diagnosis; parasitic diseases; pathologic process; polymerase chain reaction

Because the adverse reactions associated with treatment of onchocerciasis, loiasis, and lymphatic filariasis can be severe, systemic, and debilitating the mediators of this posttreatment reaction have been identified and quantified. Usually mediated, in part, by the eoinophil, the chemokines associated with eosinophil trafficking in these have been identified using immunocytochemical analysis.The clinical and immunological differences between patients who maintain (for three years) clearance of microfilaremia and those who fail to do so following definitive treatment has been assessed. The data suggest that both immunologically and clinically there are no obvious differences (save for the presence of microfilaremia) between those who remain microfilaria- free and those who are microfilaremic long term after therapy. Diagnostic Studies - PCR-based diagnostic strategies have been developed for Loa loa and Strongyloides stercoralis using recently identified repeated sequences. More importantly, a rapid format card test (based on immunochromatography) has been developed and tested; it shows sensitivities and specificities above 90 percent.

由于与盘尾丝虫病、丝虫病和淋巴丝虫病治疗相关的不良反应可能是严重的、全身性的和使人衰弱的，因此已经确定并量化了这种治疗后反应的介质。通常介导的，部分由嗜酸性粒细胞，趋化因子与嗜酸性粒细胞贩运在这些已被确定使用immunocytochemical analysis.The的临床和免疫学之间的差异保持（三年）清除微丝蚴血症和那些谁没有这样做后，明确的治疗进行了评估。 这些数据表明，在免疫学和临床上，在治疗后保持无微丝蚴的那些人和长期存在微丝蚴血症的那些人之间没有明显的差异（除了存在微丝蚴血症）。诊断研究----使用最近鉴定的重复序列，已经为Loa loa和Sternyloides stercoralis开发了基于PCR的诊断策略。更重要的是，已经开发和测试了一种快速格式卡测试（基于免疫色谱法）;它显示出90%以上的灵敏度和特异性。