Excipients for nucleic acids delivery formulations - what happens after cell entry?

将核酸（NA）的使用从疫苗转移到其他疾病的治疗将需要细胞内递送水平的阶跃变化。为此，辅料的作用至关重要。本文提出，除了NA细胞内生物利用度外，还将通过监测不同的细胞过程和“标记物”来评估制剂中所用辅料的细胞内行为和影响。国家的最先进的分子生物学技术将被用来获得这些过程的详细了解。

Moving the use of nucleic acid (NA) from vaccines to therapy of other diseases would require a step change in the level of intracellular delivery. To achieve that, the role of excipients is essential. It is proposed here that, in addition to NA intracellular bioavailability, the intracellular behaviour and effects of excipients used in formulation will be assessed by monitoring different cellular processes and 'markers'. State-of-the-art molecular biology techniques will be used to obtain a detailed understanding of these processes.