Rocky Mountain Neurological Disorders Core Center

描述(由申请人提供)： 这项申请要求为落基山神经疾病核心中心提供资金，该中心旨在为UCHSC由NINDS资助的研究人员提供服务，重点是在小鼠和斑马鱼体内使用蛋白质荧光指示剂。动物模型系统和现代显微镜技术的结合预计将导致一个核心中心，该中心将在一群已经很优秀的研究人员中催化令人兴奋的新的合作研究项目。该设施将由安吉拉·里贝拉博士领导，他将由每个核心的董事组成的指导委员会提供协助。每个调查员将根据以前对核心的使用情况来确定核心使用的优先顺序。该设施将设在UCHSC菲茨西蒙斯新校区的全新空间内。以下是单独的核心：核心A显微镜核心(比尔·贝茨，董事)将提供先进的光学和电子显微镜服务。核心B基因靶向/病毒载体核心(Mark Dell‘Acqua，董事)将设计和构建载体，以产生Gone靶向和转基因小鼠以及病毒靶向载体。Core C转基因斑马鱼Core(洛杉矶里贝拉，董事)将为转基因斑马鱼的产生和维护提供服务。CORE D机械车间(总监约翰·考德威尔)将提供机械车间和计算机服务。

DESCRIPTION (provided by applicant): This application requests funding for the Rocky Mountain Neurological Disorders Core Center, a facility with the aim of providing services for NINDS-funded investigators at UCHSC focused on the use of protein fluorescent indicators in vivo in mice and zebrafish. The combination of animal model systems and modern microscopy techniques is expected to result in a core center that will catalyze exciting new collaborative research projects within a group of already outstanding investigators. The facility will be directed by Dr. Angeles Ribera who will be assisted by a steering committee made up of the Directors of each core. Core usage will be prioritized as a function of previous usage of the core by each investigator. The facility will be housed in brand new space in the new Fitzsimons campus of the UCHSC. The following are the individual cores: Core A Microscopy Core (Bill Betz, Director) will provide advanced light and electron microscopy services. Core B Gene-Targeting/Viral Vector Core (Mark Dell'Acqua, Director) will design and construct vectors to generate gone-targeted and transgenic mice as well as viral targeting vectors. Core C Zebrafish Transgenic Core (Angeles Ribera, Director) will provide services for the generation and maintenance of transgenic zebrafish. Core D Machine Shop (John Caldwell, Director) will provide machine shop and computer services.