Rocky Mountain Neurological Disorders Core

斑马鱼核心 C3.1基本原理。核心C为RMNDC研究人员保留野生型、转基因和突变斑马鱼品系。 斑马鱼胚胎独特的发育和光学成像特性使其成为解决RMNDC研究人员研究计划核心问题的理想模型。UCD NINDS研究人员是国际公认的神经生理学，神经发育和光学成像领域的专家。 在上一个奖项期间。核心C消除了对使用模型系统感兴趣的RMNDC研究者的技术障碍（例如，贝茨、考德威尔、克劳蒂耶、尼斯万德、雷斯特雷波、泰勒、威廉姆斯） 但没有维护线路的经验或手段。 我们为核心C提出了三个具体目标，这将使RMNDC成员能够利用斑马鱼系统进行神经发育和行为研究。除了目前的13个核心C用户外，该设施还将允许那些由于与维护和繁殖斑马鱼相关的实用限制而没有考虑使用斑马鱼模型的研究人员访问斑马鱼模型。特别是， 对补充啮齿动物研究的兴趣日益增加（例如，核心B）与在斑马鱼中进行的（例如，阿佩尔、阿廷格、巴洛、克劳蒂埃、麦克林、尼斯旺德、雷斯特雷波、威廉姆斯），反之亦然。核心也将是更大的科学界的资源，因为转基因品系将在RMNDC网站上描述，并在首次出版时提供给斑马鱼国际资源中心（ZIRC）。

Zebrafish Core C3.1 Rationale. Core C maintains wild type, transgenic and mutant zebrafish strains for RMNDC investigators. The unique developmental and optical imaging properties of the zebrafish embryo makes it an ideal model to address questions at the heart of the research programs RMNDC investigators. UCD NINDS researchers are internationally recognized and expert in the fields of neurophysiology, neurodevelopment and optical imaging. During the previous award period. Core C removed technical barriers for RMNDC investigators who were interested in using the model system (e.g., Betz, Caldwell, Clouthier, Niswander, Restrepo, Taylor, Williams) but did not have experience or means for maintaining lines. We propose three Specific Aims for Core C that will allow RMNDC members to take advantage of the zebrafish system for neurodevelopmental and behavioral studies. In addition to the 13 current Core C users, the facility will allow access to the zebrafish model to investigators who have not considered its use because of the pragmatic limitations associated with maintaining and breeding zebrafish. In particular, there has been growing interest in complementing rodent studies (e.g.. Core B) with ones done in zebrafish (e.g., Appel, Artinger, Barlow, Clouthier, Macklin, Niswander, Restrepo, Williams), and vice versa. The core will also be a resource for the larger scientific community because transgenic lines will be described on the RMNDC website and made available to the Zebrafish International Resource Center (ZIRC) by the time of the first publication.