Rocky Mountain Neurological Disorders Core

描述(由申请人提供)：这是落基山神经紊乱中心(RMNOC)的竞争性更新，该中心的主要目标是加强和进一步研究尼诺斯所针对的神经生物学领域。RMNO中心核心已经建立了设施，允许高效地产生和使用表现出有趣和信息丰富的功能或行为表型的转基因动物。我们现有的核心都非常成功，并已发展为支持最先进的方法，从而使用户能够以高效的方式进行协作研究。因此，我们建议保留原始核心的原始名称，强调它们将在未来五年内继续发展，如申请中所述(核心A、6、C和0)。和以前一样，安舒茨医学院的其他地方没有这四个核心所提供的服务。 我们建议为RMNO中心核心提供四项核心设施： 核心A显微镜核心(比尔·贝茨，董事)将提供先进的光学和电子显微镜服务。 核心B基因打靶/病毒载体核心(Mark Dell‘Acqua，董事)将设计和构建载体，以产生基因打靶和转基因小鼠以及病毒打靶载体。 Core C斑马鱼转基因中心(洛杉矶里贝拉，主任)将为转基因斑马鱼的维护和鉴定提供服务。 CORE D机械车间核心(Ulli Bayer，总监)将提供机械车间支持服务。

DESCRIPTION (provided by applicant): This Is a competitive renewal of the Rocky Mountain Neurological Disorder Center (RMNOC) whose main goal Is to enhance and further research in areas of neurobiology targeted by the NINOS. The RMNO Center Core has established facilities that allow efficient generation and use of genetically-altered animals that display Interesting and Informative functional or behavioral phenotypes. Our existing cores have all been extremely successful and evolved to enable state-of-the-art approaches thereby allowing users to perform collaborative research in an efficient manner. Therefore, we propose to maintain the original cores with their original names, emphasizing that they will continue to evolve over the next five years, as described in the application (Cores A, 6, C, and 0). As before, the services provided by the four cores are not available elsewhere at the Anschutz Medical Campus. We propose four core facilities for the RMNO Center Core: Core A Microscopy Core (Bill Betz, Director) will provide advanced light and electron microscopy services. Core B Gene-Targeting/Viral Vector Core (Mark Dell'Acqua, Director) will design and construct vectors to generate gene-targeted and transgenic mice as well as viral targeting vectors. Core C Zebrafish Transgenic Core (Angeles Ribera, Director) will provide services for maintaining and characterizing transgenic zebrafish. Core D Machine Shop Core (Ulli Bayer, Director) will provide machine shop support services.