Re-Engineering Spinal Circuits to Bypass Spinal Injury

重新设计脊柱回路以绕过脊柱损伤

• 批准号: 6936826
• 负责人: LUCAS W CAMPOS
• 金额: $ 3.52万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6936826
• 关键词: antibody; axon; central nervous system; confocal scanning microscopy; gray matter; immunocytochemistry; interneurons; laboratory rat; laminectomy; microsurgery; motor neurons; nervous system regeneration; neural plasticity; neuronal guidance; peripheral nervous system; predoctoral investigator; spinal cord injury; synapses; synaptophysin

DESCRIPTION (provided by applicant): We exploit the regenerative capacity of the peripheral nervous system and the plasticity of the central nervous system (CNS) to promote recovery of motor function following spinal cord injury. A peripheral nerve is detached from its target muscle above the lesion and the cut end is inserted into the undamaged spinal cord below the lesion. We propose that novel synaptic connections will form between motor axons in the inserted nerve and the spinal neurons below the lesion. These new connections can be under voluntary supraspinal control because inputs from the brain to the regenerating motor axons are intact. Aim 1. How does the topographic distribution of regenerating T13 axons change during the period following nerve insertion? Aim 2. What is the distribution of synaptic contacts made by regenerating T13 motor axons on lumbar motoneurons and interneurons? Histology, confocal microscopy, and microsurgical techniques will be used to investigate these aims. We take one neural system which has evolved extensive regeneration capabilities, combine it with another neural system shown to be incredibly plastic and adaptive and use their combined strengths to overcome a debilitating injury.

描述(由申请人提供)： 我们利用周围神经系统的再生能力和中枢神经系统(CNS)的可塑性来促进脊髓损伤后运动功能的恢复。将周围神经从损伤上方的靶肌肉中分离出来，并将切断端插入损伤下方未损伤的脊髓中。我们认为新的突触连接将在插入的神经中的运动轴突和损伤下的脊髓神经元之间形成。这些新的连接可以在自愿的脊髓上控制，因为从大脑到再生运动轴的输入是完好无损的。 目的1.在神经植入后的一段时间内，再生T13轴突的局部分布是如何变化的？目的2.再生T13运动轴突所形成的突触接触在腰椎运动神经元和中间神经元上的分布情况如何？ 将使用组织学、共聚焦显微镜和显微外科技术来研究这些目的。我们将一个进化出广泛再生能力的神经系统与另一个被证明具有令人难以置信的可塑性和适应性的神经系统结合起来，并利用它们的组合优势来克服虚弱的伤害。