Core--Science

核心--科学

• 批准号: 6742799
• 负责人: MICHAEL A BALDWIN
• 金额: $ 21万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6742799
• 关键词: analytical method; biomedical facility; biotechnology; genetically modified animals; immunological substance; in situ hybridization; laboratory mouse; nucleic acid sequence; peptide chemical synthesis; polymerase chain reaction; prions; protein purification; recombinant proteins; tissue /cell culture; tissue /cell preparation

The Scientific Core exists to facilitate the scientific projects within the program. The services, materials and reagents provided by the Scientific Core are purified proteins from natural and recombinant sources and infectious prions, synthetic peptides, DNA sequencing, PrP antibodies, transgenic mouse screening and a chemical and biochemical analytical service. This core will be directed by Drs. Michael Baldwin and Giuseppe Legname. Dr. Baldwin, an Adjunct Professor, is an analytical chemist with extensive experience of working with peptides and proteins, and has been associated with Dr. Prusiner's prion research group for 14 years. Dr. Legname has been at UCSF for 4 years and is an Assistant Adjunct Professor with expertise in molecular biology, immunology, protein expression and biochemistry. They are already directing the Scientific Cores of other program grants in Dr. Prusiner's laboratory. Dr Giuseppe Legname will direct the preparation of recombinant PrP variants and recombinant antibodies that will be expressed in cell cultures and purified for use in the various projects, assisted by Mr. Patrick Culhane. The preparation of purified prions has been carried out in this laboratory for many years and for more than a decade has been supervised by Ms. Hana Serban, recently with assistance from Ms. Nadia Kovaleeva. The purification of infectious prions will be essential for Project 1. Ms. Serban will also supervise the production of PrP antibodies required for Projects 3 and 4 and Core C. The screening of the transgenic mice is essential for Projects 3 and 4 and will be carried by Ms. He-Ying Lin under the supervision of Dr Kurt Giles, Assistant Adjunct Professor. Peptide synthesis will be conducted by a newly appointed Assistant Adjunct Professor, who will also assist Dr. Baldwin with chemical and biochemical analysis, which will benefit all the projects. All of these activities are currently being carried out very successfully in this laboratory through the efforts of the highly experienced personnel referred to above.

科学核心的存在是为了促进该计划中的科学项目。科学核心提供的服务，材料和试剂是来自自然和重组源以及感染性王室，合成肽，DNA测序，PRP抗体，转基因小鼠筛查以及化学和生化分析服务的纯化蛋白质。该核心将由Drs执导。迈克尔·鲍德温（Michael Baldwin）和朱塞佩（Giuseppe Legname）。兼职教授Baldwin博士是一位分析化学家，拥有丰富的使用肽和蛋白质经验，并且与Prusiner博士的Prion研究小组联系了14年。 Legname博士在UCSF工作了4年，并且是一名助理兼职教授，具有分子生物学，免疫学，蛋白质表达和生物化学方面的专业知识。他们已经在Prusiner博士的实验室指导其他计划赠款的科学核心。 Giuseppe Legname博士将指导重组PRP变体和重组抗体的准备，这些变体和重组抗体将在细胞培养物中表达，并在Patrick Culhane先生的协助下纯化用于各种项目。在该实验室已经进行了多年的纯化私有私人的准备 最近在纳迪亚·科瓦里瓦（Nadia Kovaleeva）女士的协助下，哈娜·塞尔班（Hana Serban）女士对十多年的监督进行了监督。对于项目1的项目，纯化感染性毒品的净化将是必不可少的。塞尔班女士还将监督项目3和4和核心C所需的PRP抗体的生产。转基因小鼠的筛查对于项目3和4是必不可少的，并将由He-Ying-Lin女士在助理兼职教授Kurt Giles博士的监督下进行。肽合成将由新任命的助理兼职教授进行，他还将协助鲍德温博士进行化学和生化分析，这将使所有项目受益。所有这些活动目前在该实验室中都非常成功地进行 通过上述经验丰富的人员的努力。