Core--Science

科学核心的存在是为了促进该计划内的科学项目。科学核心提供的服务、材料和试剂包括从天然来源和重组来源提纯的蛋白质、传染性普恩病毒、合成肽、DNA测序、PrP抗体、转基因小鼠筛选以及化学和生化分析服务。这一核心将由迈克尔·鲍德温博士和朱塞佩·莱格曼博士执导。鲍德温博士是一名兼职教授，是一名分析化学家，在处理多肽和蛋白质方面拥有丰富的经验，并已在普鲁辛纳博士的普赖恩研究小组工作了14年。Legname博士在加州大学旧金山分校工作了4年，是一名兼职助理教授，具有分子生物学、免疫学、蛋白质表达和生物化学方面的专业知识。他们已经在普鲁辛纳博士的实验室指导其他项目拨款的科学核心。Giuseppe Legname博士将指导重组PrP变异体和重组抗体的制备工作，这些重组抗体将在细胞培养中表达，并在Patrick Culhane先生的协助下提纯用于各种项目。多年来，本实验室一直在进行纯化的普恩病毒的制备工作， 十多年来，一直由Hana Serban女士监督，最近在Nadia Kovaleeva女士的协助下。项目1的关键是提纯传染性的普恩病毒。Serban女士还将监督项目3和4以及Core C所需PrP抗体的生产。转基因小鼠的筛选对于项目3和4至关重要，将由林贺英女士在助理兼职教授Kurt Giles博士的监督下进行。多肽合成将由一名新任命的助理副教授进行，他还将协助鲍德温博士进行化学和生化分析，这将使所有项目受益。所有这些活动目前都在这个实验室非常成功地进行。 通过上文提到的经验丰富的人员的努力。

The Scientific Core exists to facilitate the scientific projects within the program. The services, materials and reagents provided by the Scientific Core are purified proteins from natural and recombinant sources and infectious prions, synthetic peptides, DNA sequencing, PrP antibodies, transgenic mouse screening and a chemical and biochemical analytical service. This core will be directed by Drs. Michael Baldwin and Giuseppe Legname. Dr. Baldwin, an Adjunct Professor, is an analytical chemist with extensive experience of working with peptides and proteins, and has been associated with Dr. Prusiner's prion research group for 14 years. Dr. Legname has been at UCSF for 4 years and is an Assistant Adjunct Professor with expertise in molecular biology, immunology, protein expression and biochemistry. They are already directing the Scientific Cores of other program grants in Dr. Prusiner's laboratory. Dr Giuseppe Legname will direct the preparation of recombinant PrP variants and recombinant antibodies that will be expressed in cell cultures and purified for use in the various projects, assisted by Mr. Patrick Culhane. The preparation of purified prions has been carried out in this laboratory for many years and for more than a decade has been supervised by Ms. Hana Serban, recently with assistance from Ms. Nadia Kovaleeva. The purification of infectious prions will be essential for Project 1. Ms. Serban will also supervise the production of PrP antibodies required for Projects 3 and 4 and Core C. The screening of the transgenic mice is essential for Projects 3 and 4 and will be carried by Ms. He-Ying Lin under the supervision of Dr Kurt Giles, Assistant Adjunct Professor. Peptide synthesis will be conducted by a newly appointed Assistant Adjunct Professor, who will also assist Dr. Baldwin with chemical and biochemical analysis, which will benefit all the projects. All of these activities are currently being carried out very successfully in this laboratory through the efforts of the highly experienced personnel referred to above.