Core--Science

科学核心的存在是为了促进计划内的科学项目。Scientific Core提供的服务、材料和试剂是来自天然和重组来源的纯化蛋白质、感染性朊病毒、合成肽、DNA测序、PrP抗体、转基因小鼠筛选以及化学和生化分析服务。这个核心将由dr。Michael Baldwin和Giuseppe Legname。Baldwin博士是一名分析化学家，在多肽和蛋白质方面拥有丰富的工作经验，并在Prusiner博士的朊病毒研究小组工作了14年。他在UCSF工作了4年，是分子生物学、免疫学、蛋白质表达和生物化学方面的助理兼职教授。他们已经在指导普鲁西纳实验室其他项目的科学核心。Giuseppe Legname博士将指导重组PrP变体和重组抗体的制备，这些变体和抗体将在细胞培养中表达，并在Patrick Culhane先生的协助下进行纯化，以供各种项目使用。纯化朊病毒的制备已经在这个实验室进行了多年，并为

The Scientific Core exists to facilitate the scientific projects within the program. The services, materials and reagents provided by the Scientific Core are purified proteins from natural and recombinant sources and infectious prions, synthetic peptides, DNA sequencing, PrP antibodies, transgenic mouse screening and a chemical and biochemical analytical service. This core will be directed by Drs. Michael Baldwin and Giuseppe Legname. Dr. Baldwin, an Adjunct Professor, is an analytical chemist with extensive experience of working with peptides and proteins, and has been associated with Dr. Prusiner's prion research group for 14 years. Dr. Legname has been at UCSF for 4 years and is an Assistant Adjunct Professor with expertise in molecular biology, immunology, protein expression and biochemistry. They are already directing the Scientific Cores of other program grants in Dr. Prusiner's laboratory. Dr Giuseppe Legname will direct the preparation of recombinant PrP variants and recombinant antibodies that will be expressed in cell cultures and purified for use in the various projects, assisted by Mr. Patrick Culhane. The preparation of purified prions has been carried out in this laboratory for many years and for more than a decade has been supervised by Ms. Hana Serban, recently with assistance from Ms. Nadia Kovaleeva. The purification of infectious prions will be essential for Project 1. Ms. Serban will also supervise the production of PrP antibodies required for Projects 3 and 4 and Core C. The screening of the transgenic mice is essential for Projects 3 and 4 and will be carried by Ms. He-Ying Lin under the supervision of Dr Kurt Giles, Assistant Adjunct Professor. Peptide synthesis will be conducted by a newly appointed Assistant Adjunct Professor, who will also assist Dr. Baldwin with chemical and biochemical analysis, which will benefit all the projects. All of these activities are currently being carried out very successfully in this laboratory through the efforts of the highly experienced personnel referred to above.