Core--Science

科学核心的存在是为了促进该计划内的科学项目。Scientific Core提供的服务、材料和试剂包括天然和重组来源的纯化蛋白质、传染性朊病毒、合成肽、DNA测序、PrP抗体、转基因小鼠筛选以及化学和生化分析服务。该核心将由Michael Baldwin博士和Giuseppe Legname博士指导。Baldwin博士是一名兼职教授，是一名分析化学家，在肽和蛋白质方面拥有丰富的工作经验，并与Prusiner博士的朊病毒研究小组合作了14年。Legname博士在UCSF工作了4年，是一名助理兼职教授，在分子生物学、免疫学、蛋白质表达和生物化学方面具有专业知识。他们已经在Prusiner博士的实验室指导其他项目赠款的科学核心。Giuseppe Legname博士将指导重组PrP变体和重组抗体的制备，这些变体和抗体将在细胞培养物中表达，并在帕特里克Culhane先生的协助下纯化，用于各种项目。纯化朊病毒的制备已在该实验室进行多年， 十多年来，该中心一直由Hana Serban女士监督，最近得到了Nadia Kovaleeva女士的协助。传染性朊病毒的纯化对项目1至关重要。Serban女士还将监督项目3和4以及核心C所需的PrP抗体的生产。转基因小鼠的筛选是项目3和4的关键，将由林和英女士在助理兼职教授Kurt Giles博士的监督下进行。肽合成将由一位新任命的助理兼职教授进行，他还将协助鲍德温博士进行化学和生化分析，这将使所有项目受益。所有这些活动目前都在这个实验室非常成功地进行 通过上述经验丰富的人员的努力，

The Scientific Core exists to facilitate the scientific projects within the program. The services, materials and reagents provided by the Scientific Core are purified proteins from natural and recombinant sources and infectious prions, synthetic peptides, DNA sequencing, PrP antibodies, transgenic mouse screening and a chemical and biochemical analytical service. This core will be directed by Drs. Michael Baldwin and Giuseppe Legname. Dr. Baldwin, an Adjunct Professor, is an analytical chemist with extensive experience of working with peptides and proteins, and has been associated with Dr. Prusiner's prion research group for 14 years. Dr. Legname has been at UCSF for 4 years and is an Assistant Adjunct Professor with expertise in molecular biology, immunology, protein expression and biochemistry. They are already directing the Scientific Cores of other program grants in Dr. Prusiner's laboratory. Dr Giuseppe Legname will direct the preparation of recombinant PrP variants and recombinant antibodies that will be expressed in cell cultures and purified for use in the various projects, assisted by Mr. Patrick Culhane. The preparation of purified prions has been carried out in this laboratory for many years and for more than a decade has been supervised by Ms. Hana Serban, recently with assistance from Ms. Nadia Kovaleeva. The purification of infectious prions will be essential for Project 1. Ms. Serban will also supervise the production of PrP antibodies required for Projects 3 and 4 and Core C. The screening of the transgenic mice is essential for Projects 3 and 4 and will be carried by Ms. He-Ying Lin under the supervision of Dr Kurt Giles, Assistant Adjunct Professor. Peptide synthesis will be conducted by a newly appointed Assistant Adjunct Professor, who will also assist Dr. Baldwin with chemical and biochemical analysis, which will benefit all the projects. All of these activities are currently being carried out very successfully in this laboratory through the efforts of the highly experienced personnel referred to above.