Core--Science

核心--科学

• 批准号: 6742799
• 负责人: MICHAEL A BALDWIN
• 金额: $ 21万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6742799
• 关键词: analytical method; biomedical facility; biotechnology; genetically modified animals; immunological substance; in situ hybridization; laboratory mouse; nucleic acid sequence; peptide chemical synthesis; polymerase chain reaction; prions; protein purification; recombinant proteins; tissue /cell culture; tissue /cell preparation

The Scientific Core exists to facilitate the scientific projects within the program. The services, materials and reagents provided by the Scientific Core are purified proteins from natural and recombinant sources and infectious prions, synthetic peptides, DNA sequencing, PrP antibodies, transgenic mouse screening and a chemical and biochemical analytical service. This core will be directed by Drs. Michael Baldwin and Giuseppe Legname. Dr. Baldwin, an Adjunct Professor, is an analytical chemist with extensive experience of working with peptides and proteins, and has been associated with Dr. Prusiner's prion research group for 14 years. Dr. Legname has been at UCSF for 4 years and is an Assistant Adjunct Professor with expertise in molecular biology, immunology, protein expression and biochemistry. They are already directing the Scientific Cores of other program grants in Dr. Prusiner's laboratory. Dr Giuseppe Legname will direct the preparation of recombinant PrP variants and recombinant antibodies that will be expressed in cell cultures and purified for use in the various projects, assisted by Mr. Patrick Culhane. The preparation of purified prions has been carried out in this laboratory for many years and for more than a decade has been supervised by Ms. Hana Serban, recently with assistance from Ms. Nadia Kovaleeva. The purification of infectious prions will be essential for Project 1. Ms. Serban will also supervise the production of PrP antibodies required for Projects 3 and 4 and Core C. The screening of the transgenic mice is essential for Projects 3 and 4 and will be carried by Ms. He-Ying Lin under the supervision of Dr Kurt Giles, Assistant Adjunct Professor. Peptide synthesis will be conducted by a newly appointed Assistant Adjunct Professor, who will also assist Dr. Baldwin with chemical and biochemical analysis, which will benefit all the projects. All of these activities are currently being carried out very successfully in this laboratory through the efforts of the highly experienced personnel referred to above.

科学核心的存在是为了促进该计划内的科学项目。科学核心提供的服务、材料和试剂包括来自天然和重组来源的纯化蛋白质和传染性朊病毒、合成肽、DNA 测序、PrP 抗体、转基因小鼠筛选以及化学和生化分析服务。该核心将由博士指导。迈克尔·鲍德温和朱塞佩·莱格纳姆。 Baldwin 博士是一位兼职教授，也是一位分析化学家，在肽和蛋白质研究方面拥有丰富的经验，并与 Prusiner 博士的朊病毒研究小组合作了 14 年。 Legname 博士已在 UCSF 工作 4 年，是一名助理兼职教授，拥有分子生物学、免疫学、蛋白质表达和生物化学方面的专业知识。他们已经在指导 Prusiner 博士实验室的其他项目资助的科学核心。 Giuseppe Legname 博士将在 Patrick Culhane 先生的协助下指导重组 PrP 变体和重组抗体的制备，这些变体和重组抗体将在细胞培养物中表达并纯化以用于各种项目。该实验室多年来一直致力于纯化朊病毒的制备 十多年来，一直由 Hana Serban 女士监督，最近在 Nadia Kovaleeva 女士的协助下。传染性朊病毒的纯化对于项目 1 至关重要。Serban 女士还将监督项目 3 和 4 以及核心 C 所需的 PrP 抗体的生产。转基因小鼠的筛选对于项目 3 和 4 至关重要，并将由 He-Ying Lin 女士在助理教授 Kurt Giles 博士的监督下进行。肽合成将由新任命的助理兼职教授进行，他还将协助鲍德温博士进行化学和生化分析，这将使所有项目受益。所有这些活动目前正在该实验室中非常成功地进行 通过上述经验丰富的人员的努力。