CORE--SCIENTIFIC

核心--科学

• 批准号: 6112237
• 负责人: MICHAEL A BALDWIN
• 金额: $ 18.35万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-07-01 至 2000-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6112237
• 关键词: SDS polyacrylamide gel electrophoresis; animal extract; biomedical facility; circular dichroism; fluorescence spectrometry; genetically modified animals; high performance liquid chromatography; immunological substance; in situ hybridization; infrared spectrometry; interferometry; laboratory mouse; mass spectrometry; nucleic acid chemical synthesis; nucleic acid sequence; oligonucleotides; peptide chemical synthesis; polymerase chain reaction; prions; protein purification; protein sequence; ultracentrifugation

The Scientific Core is a modest unit set up to facilitate the scientific projects within the program. The services and reagents provided by the Scientific Core are purified prions, bioassays, antibodies, synthetic peptides, and neuropathologic evaluations. The bioassay part of the Scientific Core obviously overlaps with the Animal Core. Most of the projects require purified prions, bioassays, antibodies to PrP peptides, and neuropathologic evaluations at least of selected animals. Two of the projects also use synthetic peptides. To provide these services and reagents, Dr. Stephen Dearmond, a skilled neuropathologist, and Dr. Dan Serban, a talented immunologist, are needed. In addition, the skills of Ms. Hana Serban and Ms. Darlene Groth are crucial to the smooth operation of the Scientific Core and the provision of purified prions.

科学核心是为促进科学发展而设立的一个适度的单位 计划内的项目。本组织提供的服务和试剂 科学核心是纯化的朊病毒、生物分析、抗体、人工合成 多肽和神经病理评估。生物测定部分 科学核心显然与动物核心重叠。大多数 项目需要纯化的普恩病毒，生物检测，PrP多肽抗体， 以及至少对选定动物的神经病理学评估。其中两个 项目也使用合成肽。为了提供这些服务和 试剂，经验丰富的神经病理学家斯蒂芬·迪尔蒙德博士和丹博士 需要一位才华横溢的免疫学家瑟班。此外，他们的技能还包括 Hana Serban女士和Darlene Groth女士对顺利运作至关重要 科学核心和提供纯化的普恩病毒。