EXPLOSIVE MATRIX ASSISTED LASER DESORPTION IONIZATION MASS SPECTROMETRY

爆炸基质辅助激光解吸电离质谱法

• 批准号: 6120204
• 负责人: MICHAEL A BALDWIN
• 金额: $ 6.2万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-03-01 至 2000-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6120204
• 关键词: biological products; biomedical resource; proteins; spectrometry; structural biology

Mass spectrometry has become an essential technique for the analysis of biological macromolecules such as proteins and nucleic acids. Ionization techniques for involatile materials are key to the success of mass spectrometry in biology and medicine. Matrix assisted laser desorption/ionization (MALDI) is one such method. Further development of this technique will be dependent upon the identification of new matrix materials with properties of a type that have not been exploited before now. We propose to investigate the use of explosives as new matrices that will impart additional energy to the analyte upon laser irradiation, thereby increasing the sensitivity of detection. This will require the systematic study of the interactions between potential matrices and biopolymers. The UCSF Mass Spectrometry Facility will provide access to MALDI instruments for these studies and will thereby be critical to the successful outcome.

质谱分析已成为一种基本的技术， 生物大分子如蛋白质和核酸的分析 acids. 不挥发性物质的电离技术是 质谱在生物学和医学上的成功。 基质辅助 激光解吸/电离（MALDI）就是这样一种方法。 进一步 这项技术的发展将取决于 识别具有如下性质的新型基质材料， 以前从未被利用过。 我们建议调查 爆炸物作为新的矩阵，将赋予额外的能量， 分析物，从而增加灵敏度 的检测。 这将需要系统地研究 潜在基质和生物聚合物之间的相互作用。 大学旧金山 质谱设施将提供使用MALDI仪器的机会 这些研究，从而将是至关重要的成功 结果。