EXPLOSIVE MATRIX ASSISTED LASER DESORPTION IONIZATION MASS SPECTROMETRY

质谱学已经成为一项基本的技术 蛋白质、核酸等生物大分子的分析 酸。不稳定材料的电离技术是 质谱学在生物和医学领域的成功。矩阵辅助 激光解吸/电离(MALDI)就是这样一种方法。进一步 这项技术的发展将取决于 具有以下类型的性能的新基质材料的鉴定 在此之前从未被开发过。我们建议对其使用情况进行调查 炸药作为新的基质，将提供额外的能量 激光照射下的分析物，从而提高了灵敏度 关于侦测的问题。这将需要对 潜在基质和生物聚合物之间的相互作用。加州大学旧金山分校 质谱仪将提供对MALDI仪器的访问 对于这些研究来说，并因此将是成功的关键 结果。

Mass spectrometry has become an essential technique for the analysis of biological macromolecules such as proteins and nucleic acids. Ionization techniques for involatile materials are key to the success of mass spectrometry in biology and medicine. Matrix assisted laser desorption/ionization (MALDI) is one such method. Further development of this technique will be dependent upon the identification of new matrix materials with properties of a type that have not been exploited before now. We propose to investigate the use of explosives as new matrices that will impart additional energy to the analyte upon laser irradiation, thereby increasing the sensitivity of detection. This will require the systematic study of the interactions between potential matrices and biopolymers. The UCSF Mass Spectrometry Facility will provide access to MALDI instruments for these studies and will thereby be critical to the successful outcome.