EXPLOSIVE MATRIX ASSISTED LASER DESORPTION IONIZATION MASS SPECTROMETRY

爆炸基质辅助激光解吸电离质谱法

• 批准号: 6120204
• 负责人: MICHAEL A BALDWIN
• 金额: $ 6.2万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-03-01 至 2000-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6120204
• 关键词: biological products; biomedical resource; proteins; spectrometry; structural biology

Mass spectrometry has become an essential technique for the analysis of biological macromolecules such as proteins and nucleic acids. Ionization techniques for involatile materials are key to the success of mass spectrometry in biology and medicine. Matrix assisted laser desorption/ionization (MALDI) is one such method. Further development of this technique will be dependent upon the identification of new matrix materials with properties of a type that have not been exploited before now. We propose to investigate the use of explosives as new matrices that will impart additional energy to the analyte upon laser irradiation, thereby increasing the sensitivity of detection. This will require the systematic study of the interactions between potential matrices and biopolymers. The UCSF Mass Spectrometry Facility will provide access to MALDI instruments for these studies and will thereby be critical to the successful outcome.

质谱分析已成为重要的技术 蛋白质、核酸等生物大分子分析 酸。 不挥发性材料的电离技术是关键 质谱在生物学和医学领域的成功。 矩阵辅助 激光解吸/电离（MALDI）就是这样的一种方法。 更远 这项技术的发展将取决于 鉴定具有以下性质的新基质材料 之前没有被利用过。 我们建议调查使用 炸药作为新的基质，将赋予额外的能量 激光照射下的分析物，从而提高灵敏度 的检测。 这需要系统地研究 潜在基质和生物聚合物之间的相互作用。 加州大学旧金山分校 质谱设施将提供 MALDI 仪器 对于这些研究来说，因此对于成功至关重要 结果。