Genomic analyses of autism spectrum disorders

自闭症谱系障碍的基因组分析

• 批准号: 7142718
• 负责人: VALERIE W HU
• 金额: $ 20.64万
• 依托单位: GEORGE WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7142718
• 关键词: autism; biomarker; cell line; family genetics; gene expression; genetic disorder diagnosis; genetic library; genetic mapping; genetic screening; human tissue; information systems; interview; linkage mapping; lymphoblast; microarray technology; patient oriented research; phenotype; polymerase chain reaction; quantitative trait loci; questionnaires; tissue resource /registry

DESCRIPTION (provided by applicant): The twin goals of this study are to use large-scale genomic methods to identify biomarkers of autism spectrum disorders (ASD) in cell lines derived from autistic individuals, and to gain a better understanding of the biology of these disorders. The central hypothesis driving this analytical approach is that blood cells from individuals with ASD will reflect molecular defects or genetic control elements that are relevant to autism. This hypothesis and approach is supported by of our preliminary findings: 1) cell lines derived from the blood of three identical twin pairs that differ in severity of ASD show a different profile of expressed genes; 2) the shared highly differentially expressed genes are significantly enriched in pathways critical to the development and function of the nervous system; 3) the levels of expression of certain genes appear to be related to the severity of the disorder when compared to the levels of expression of the same genes in cell lines from respective non-affected siblings; 4) candidate genes from preliminary microarray studies have associated quantitative trait loci containing reported autism susceptibility genes or loci. Thus, this differential expression profile which is observed in easily accessible blood-derived cells may be reflective of aberrant gene expression in the autistic vs. normal brain. This study will utilize DNA microarrays to: 1) identify differentially expressed genes in lymphoblastoid cell lines from individuals with ASD in comparison to unaffected individuals; 2) determine whether subgroups of ASD segregated according to phenotypic expression of ASD using existing diagnostic instruments, such as the ADI-R, can be differentiated through gene expression profiling; 3) build a classifier for ASD based on various class prediction algorithms, including k-nearest neighbors, centroid classification, and neural networks; 4) analyze signaling or metabolic pathways affected in the experimental subgroups; 5) map and identify genetic determinants that are responsible for differentially expressed genes using existing genetic data in the Autism Genetics Resource Exchange genotype database. At present, diagnosis of ASD relies primarily upon sometimes biased behavioral observations by clinicians or therapists and parent/teacher questionnaires. Reliable biomarkers would greatly facilitate the early and definitive detection of these disorders, thereby permitting early intervention and therapy.

描述(申请人提供)：这项研究的双重目标是使用大规模基因组方法在自闭症患者的细胞系中鉴定自闭症谱系障碍(ASD)的生物标记物，并更好地了解这些疾病的生物学。推动这一分析方法的中心假设是，自闭症患者的血细胞将反映与自闭症相关的分子缺陷或遗传控制因素。这一假设和方法得到了我们的初步发现的支持：1)来自三对严重程度不同的ASD同卵双胞胎的细胞系显示了不同的表达基因谱；2)共享的高度差异表达的基因在对神经系统的发育和功能至关重要的通路中得到了显著的丰富；3)某些基因的表达水平似乎与疾病的严重程度有关，当与来自各自未受影响的兄弟姐妹的细胞系中相同基因的表达水平进行比较时；4)来自初步微阵列研究的候选基因具有相关的包含自闭症易感基因或基因座的相关数量性状基因座。因此，在易于获取的血源性细胞中观察到的这种差异表达谱可能反映了自闭症患者与正常大脑中基因表达的异常。这项研究将利用DNA微阵列来：1)找出ASD患者和非ASD患者之间的差异表达基因；2)确定是否可以通过基因表达谱等现有诊断工具，如ADI-R，根据ASD的表型表达分离出ASD的亚组；3)基于各种类别预测算法，包括K近邻、质心分类和神经网络，构建ASD的分类器；4)分析实验亚组中受影响的信号或代谢通路；5)使用自闭症遗传学资源交换基因数据库中的现有遗传数据，定位和鉴定与ASD差异表达基因有关的遗传决定因素。目前，ASD的诊断主要依赖于临床医生或治疗师有时有偏见的行为观察和家长/教师问卷。可靠的生物标志物将极大地促进这些疾病的早期和明确检测，从而使早期干预和治疗成为可能。