A Novel Device for Determination of GFR Markers

一种测定 GFR 标志物的新型装置

• 批准号: 6935554
• 负责人: BIKAS VAIDYA
• 金额: $ 14.57万
• 依托单位: LYNNTECH, INC.
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-08-01 至 2006-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6935554
• 关键词: biomedical equipment development; blood chemistry; calorimetry; clinical biomedical equipment; clinical research; electrochemistry; glomerular filtration rate; high performance liquid chromatography; human tissue; iodine; kidney disorder diagnosis; urinalysis

DESCRIPTION (provided by applicant): About 20 million Americans have chronic kidney disease while an additional 20 million are at risk. Glomerular Filtration Rate (GFR) is one of the best indexes of kidney function. It is determined by measuring the rate of disappearance of certain markers from blood and their appearance in the urine. Currently used methods for measuring the GFR are either expensive, difficult, or require radioactive markers, and specialized facility and trained personnel. Thus, there is a great need to develop a convenient and rapid method for GFR measurement that does not require use of radioactive markers, is accurate and costeffective. In order to satisfy this need, Lynntech is proposing the development of a simple device to measure GFR using non-radioactive iodinated markers such as iothalamate. This device is based on an innovative method that combines electrochemistry, solid phase extraction (SPE) and colorimetry. During Phase I of this project, Lynntech will investigate the feasibility of using this innovative combination of electrochemistry in conjunction with SPE and colorimetry for accurate determination of GFR. This method will be tested with blood/serum and urine samples and the results compared to that obtained using standard HPLC method for determination of the GFR markers at Indiana University. The optimized conditions obtained during the Phase I will be used for designing, fabricating and testing of a prototype device for rapid and accurate measurement of GFR during Phase II.

描述（由申请人提供）：大约2000万美国人患有慢性肾脏疾病，另有2000万人处于危险之中。肾小球滤过率（Glomerular Filtration Rate， GFR）是肾功能的最佳指标之一。它是通过测量某些标记物从血液中消失的速度和它们在尿液中的出现来确定的。目前使用的测量GFR的方法要么昂贵，要么困难，要么需要放射性标记，以及专门的设备和训练有素的人员。因此，非常需要开发一种方便快速的GFR测量方法，该方法不需要使用放射性标记物，既准确又具有成本效益。为了满足这一需求，Lynntech建议开发一种简单的设备，使用非放射性碘化标记物（如碘甲酸酯）来测量GFR。该装置基于一种结合了电化学、固相萃取（SPE）和比色法的创新方法。在该项目的第一阶段，Lynntech将研究使用电化学与SPE和比色法相结合的创新组合来准确测定GFR的可行性。该方法将在血液/血清和尿液样本中进行测试，并将结果与印第安纳大学使用标准HPLC法测定GFR标记物的结果进行比较。第一阶段获得的优化条件将用于第二阶段快速准确测量GFR的原型设备的设计、制造和测试。