A Novel Device for Determination of GFR Markers

一种测定 GFR 标志物的新型装置

• 批准号: 6935554
• 负责人: BIKAS VAIDYA
• 金额: $ 14.57万
• 依托单位: LYNNTECH, INC.
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-08-01 至 2006-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6935554
• 关键词: biomedical equipment development; blood chemistry; calorimetry; clinical biomedical equipment; clinical research; electrochemistry; glomerular filtration rate; high performance liquid chromatography; human tissue; iodine; kidney disorder diagnosis; urinalysis

DESCRIPTION (provided by applicant): About 20 million Americans have chronic kidney disease while an additional 20 million are at risk. Glomerular Filtration Rate (GFR) is one of the best indexes of kidney function. It is determined by measuring the rate of disappearance of certain markers from blood and their appearance in the urine. Currently used methods for measuring the GFR are either expensive, difficult, or require radioactive markers, and specialized facility and trained personnel. Thus, there is a great need to develop a convenient and rapid method for GFR measurement that does not require use of radioactive markers, is accurate and costeffective. In order to satisfy this need, Lynntech is proposing the development of a simple device to measure GFR using non-radioactive iodinated markers such as iothalamate. This device is based on an innovative method that combines electrochemistry, solid phase extraction (SPE) and colorimetry. During Phase I of this project, Lynntech will investigate the feasibility of using this innovative combination of electrochemistry in conjunction with SPE and colorimetry for accurate determination of GFR. This method will be tested with blood/serum and urine samples and the results compared to that obtained using standard HPLC method for determination of the GFR markers at Indiana University. The optimized conditions obtained during the Phase I will be used for designing, fabricating and testing of a prototype device for rapid and accurate measurement of GFR during Phase II.

描述（由申请人提供）：约有2000万美国人患有慢性肾脏疾病，而另外2000万人处于危险之中。肾小球滤过率（GFR）是肾功能的最佳指数之一。它是通过测量血液中某些标记的消失率及其在尿液中的外观来确定的。目前使用的测量GFR的方法要么昂贵，困难，要么需要放射性标记，并且需要专门的设施和训练有素的人员。因此，非常需要开发一种不需要使用放射性标记的GFR测量方便而快速的方法，这是准确且具有成本效益的。为了满足这一需求，Lynntech提出了使用非放射性碘标记（例如Iothalamate）来测量GFR的开发，以测量GFR。该设备基于一种创新方法，该方法结合了电化学，固相提取（SPE）和比色法。在该项目的第一阶段期间，Lynntech将研究使用这种电化学与SPE和比色的创新组合以准确确定GFR的可行性。该方法将通过血/血清和尿液样本进行测试，与使用标准HPLC方法获得的结果相比，该方法的结果是确定印第安纳大学的GFR标记的结果。在阶段I期间获得的优化条件将用于设计，制造和测试原型设备，以快速准确地测量II期GFR。