Biomagnetic Determinantion of Transplant Rejection

移植排斥的生物磁测定

• 批准号: 6989919
• 负责人: EDWARD R FLYNN
• 金额: $ 10万
• 依托单位: SENIOR SCIENTIFIC
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-15 至 2006-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6989919
• 关键词: biosensor device; biotechnology; cytotoxic T lymphocyte; diagnosis design /evaluation; immunopathology diagnosis; kidney transplantation; magnetic field; model design /development; nanotechnology; noninvasive diagnosis; particle; phantom model; surface coating; transplant rejection

DESCRIPTION (provided by applicant): A significant percentage of organ transplants are rejected by the immune system. Symptoms of rejection vary depending upon the organ but a biopsy of the transplant is normally required to ascertain that rejection is indeed happening. Biopsies are painful, introduce the possibility of infection, and require subsequent microscopic inspection of the biopsy tissue for lymphocytes. Acute rejection occurs through several mechanisms, but is dominated by multiplication of T cells which attach to HLA antigens on the donor cells and kill them. It is possible to identify the presence of these T cell lymphocytes in the transplanted organ by non-invasive biomagnetic techniques using introduced magnetic nanoparticles specifically targeted to lymphocytes by antibodies, such as CD8+, which attach to their ligand receptors. Sensitive SQUID magnetic sensors can detect the presence of small amounts of these labeled cells by magnetizing the nanoparticles and subsequent measurement of their remanence fields. This methodology can be used to monitor the status of a transplant for the effects of chemotherapy as well as reduce the need for biopsies.

描述（由申请人提供）：很大比例的器官移植被免疫系统排斥。排斥反应的症状因器官而异，但通常需要对移植进行活检以确定排斥反应确实发生。活检是痛苦的，引入感染的可能性，并且需要随后对活检组织进行淋巴细胞的显微镜检查。急性排斥通过几种机制发生，但主要是T细胞的增殖，T细胞附着在供体细胞上的HLA抗原上并杀死它们。有可能通过非侵入性生物磁性技术鉴定移植器官中这些T细胞淋巴细胞的存在，该技术使用通过抗体（例如CD8+）特异性靶向淋巴细胞的引入的磁性纳米颗粒，所述抗体附着于其配体受体。敏感的SQUID磁传感器可以通过磁化纳米颗粒并随后测量其剩磁场来检测少量这些标记细胞的存在。这种方法可用于监测移植的化疗效果，并减少活检的需要。