Biomagnetic Determinantion of Transplant Rejection

移植排斥的生物磁测定

• 批准号: 6989919
• 负责人: EDWARD R FLYNN
• 金额: $ 10万
• 依托单位: SENIOR SCIENTIFIC
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-15 至 2006-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6989919
• 关键词: biosensor device; biotechnology; cytotoxic T lymphocyte; diagnosis design /evaluation; immunopathology diagnosis; kidney transplantation; magnetic field; model design /development; nanotechnology; noninvasive diagnosis; particle; phantom model; surface coating; transplant rejection

DESCRIPTION (provided by applicant): A significant percentage of organ transplants are rejected by the immune system. Symptoms of rejection vary depending upon the organ but a biopsy of the transplant is normally required to ascertain that rejection is indeed happening. Biopsies are painful, introduce the possibility of infection, and require subsequent microscopic inspection of the biopsy tissue for lymphocytes. Acute rejection occurs through several mechanisms, but is dominated by multiplication of T cells which attach to HLA antigens on the donor cells and kill them. It is possible to identify the presence of these T cell lymphocytes in the transplanted organ by non-invasive biomagnetic techniques using introduced magnetic nanoparticles specifically targeted to lymphocytes by antibodies, such as CD8+, which attach to their ligand receptors. Sensitive SQUID magnetic sensors can detect the presence of small amounts of these labeled cells by magnetizing the nanoparticles and subsequent measurement of their remanence fields. This methodology can be used to monitor the status of a transplant for the effects of chemotherapy as well as reduce the need for biopsies.

描述（由申请人提供）：免疫系统拒绝了很大比例的器官移植物。排斥反应的症状因器官而异，但通常需要对移植的活检才能确定拒绝确实正在发生。活检是痛苦的，引入了感染的可能性，需要对活检组织进行淋巴细胞的随后显微镜检查。急性排斥是通过几种机制发生的，但由T细胞的繁殖支配，这些T细胞附着于HLA抗原上的供体细胞上并杀死它们。使用非侵入性生物磁磁技术在移植器官中的存在，使用引入的磁性纳米颗粒，这些磁性纳米颗粒是通过抗体（例如CD8+）靶向淋巴细胞的。敏感的鱿鱼磁传感器可以通过磁化纳米颗粒并随后测量其remanence场来检测少量标记的细胞的存在。该方法可用于监测化学疗法影响的移植状态，并减少对活检的需求。