Improving Biopsies using Magnetic Nanoparticles

描述(申请人提供)：对于患有急性淋巴细胞性白血病(ALL)和老年患者的骨髓增生异常综合征(MDS)的儿童，在早期阶段测量微小残留病(MRD)以监测化疗进展是极其重要的，这种测量决定了预后、疗效和治疗水平。通常的检测和监测方法是通过骨髓活检。不幸的是，这种方法需要多次活检才能获得足够的样本，而且这些样本必须定期进行，以监测治疗情况。通过使用适当标记的磁性纳米颗粒和使用磁性收集方法，可以显著提高活检的效率。这些抗体标记的磁性纳米颗粒寻找并附着在癌细胞上，然后这些癌细胞可以被磁性收集。本赠款申请描述了使用磁性活检针的活组织检查技术背后的方法，这使得有可能大幅提高活组织检查的效率以及对特定白血病癌症类型的特异性，但也包括对骨髓中转移的罕见癌细胞的采样。通过使用涂有抗癌剂和磁浓缩的磁性纳米颗粒，可以在癌症部位进行直接治疗干预。公共卫生相关性：用于疾病检测的纳米技术、用于靶向疾病的磁性纳米颗粒、用于骨髓活检的磁针

DESCRIPTION (provided by applicant): In children with acute lymphomatic leukemia (ALL) and in myelodysplastic syndrome (MDS) with older patients it is extremely important to measure the minimal residual disease (MRD) to monitor the progress of chemotherapy at early stages Such measurements determine the prognosis, efficacy, and levels of the treatment. The normal method for detection and monitoring is through bone marrow biopsies. Unfortunately, this method requires multiple biopsies to obtain sufficient samples and these must be made at periodic intervals to monitor the treatment. It is possible to substantially improve the efficiency of biopsies through the use of appropriately labeled magnetic nanoparticles and using magnetic collection methods. These antibody-labeled magnetic nanoparticles seek out, and attach themselves to, cancer cells, which can then be collected magnetically. This grant application describes the methodology behind this biopsy technique using a }smart} magnetic biopsy needle making it possible to substantially increase the efficiency of the biopsy as well as the specificity for particular leukemia cancer types but also including the sampling of metastatic rare cancer cells in the marrow. By using magnetic nanoparticles coated with anti-cancer agents and magnetically concentrated, direct therapeutic intervention at the cancer site is possible. PUBLIC HEALTH RELEVANCE: Nanotechnology for Disease Detection, Magnetic Nanoparticles for targeting Disease, Magnetic Needle for Bone Marrow Biopsy