CLINICAL IMMUNOLOGY LABORATORY

临床免疫学实验室

• 批准号: 7391969
• 负责人: Peter J Felsburg
• 金额: $ 3.36万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-01 至 2007-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7391969
• 关键词: CLINICAL; IMMUNOLOGY; LABORATORY

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Inherited immunodeficiency disorders are an important class of genetic disease. Dr. Peter Felsburg has instituted a full-service clinical immunology laboratory for dogs and cats at the Veterinary School, University of Pennsylvania, which will aid the screening laboratory in identifying companion animals with primary immunodeficiency diseases. The testing provided by the laboratory includes flow cytometry to determine in dogs: CD3, CD4, CD8, CD14, CD21, CD45RA, TCRa¿, and TCRgd; in cats: CD5, CD4, CD8, CD14, and CD21. In addition, a panel to detect defective leukocyte integrin expression includes CD11a, CD11b, CD11c, and CD18. We also now offer serum immunoglobulin quantitation (IgG, IgM, and IgA), a lymphocyte proliferation assay, and a neutrophil/monocyte function test that measures hydrogen peroxide production. With this battery of tests, we will be better able to identify genetic abnormalities in immune function, expanding the capacity to uncover homologes of human genetic disease.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中得到体现。列出的机构是中心的机构，不一定是研究者的机构。遗传性免疫缺陷病是一类重要的遗传病。 Peter Felsburg 博士在宾夕法尼亚大学兽医学院建立了一个为狗和猫提供全方位服务的临床免疫学实验室，该实验室将帮助筛查实验室识别患有原发性免疫缺陷疾病的伴侣动物。 实验室提供的检测包括流式细胞术，以确定狗的 CD3、CD4、CD8、CD14、CD21、CD45RA、TCRa¿ 和 TCRgd；猫：CD5、CD4、CD8、CD14 和 CD21。此外，检测缺陷白细胞整合素表达的一组包括 CD11a、CD11b、CD11c 和 CD18。我们现在还提供血清免疫球蛋白定量（IgG、IgM 和 IgA）、淋巴细胞增殖测定以及测量过氧化氢产生的中性粒细胞/单核细胞功能测试。通过这一系列测试，我们将能够更好地识别免疫功能的遗传异常，从而扩大发现人类遗传疾病同源物的能力。