Core--Biochemistry and Signal Transduction

核心--生物化学与信号转导

• 批准号: 7122650
• 负责人: MICHAEL G. ZIEGLER
• 金额: $ 16.09万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-06-01 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7122650
• 关键词: adrenergic receptor; bioassay; biochemistry; biological signal transduction; biomedical facility; catecholamines; enzyme activity; epinephrine; immunocytochemistry; neuropeptide Y; neurotransmitters; radioimmunoassay; second messengers

The Biochemistry Core and Signal Transduction Core were separate units. They have been combined in this renewal application since they utilize similar methods and utilize personnel who can perform some of the assays in both cores. In addition, part of the efforts of the Signal Transduction Core during the current funding period involved development of methods and identification of genetic variants in receptors and other signaling molecules. These efforts will now be undertaken by Core D. The combination of the Biochemistry and Signal Transduction Cores will permit more efficient operation by decreasing the combined effort of the core directors from 10% to 8% and by allowing personnel to combine efforts. The resulting efficiencies are reflected in the budget, which is less than the combined budgets of the two cores currently in operation. Although the total number of assays performed by the cores will not decrease, laboratory personnel are now more familiar with performing high volume assays, and can thus carry them out more efficiently. Unfortunately, supplies for these assays have not gone down in price, so we have not been able to decrease the supply budget as much as the personnel budget.

生化核心和信号传导核心是两个独立的单元。在本次更新申请中将其合并，因为它们采用相似的方法，并利用可以在两个核心中执行某些测定的人员。此外，信号转导核心在当前资助期间的部分工作涉及开发方法和识别受体和其他信号分子的遗传变异。这些工作现在将由核心D承担。生物化学和信号转导核心的组合将使核心主管的综合工作量从10%减少到8%，并允许工作人员联合收割机工作，从而实现更有效的操作。由此产生的效率反映在预算中，低于目前运作的两个核心的合并预算。尽管由该公司进行的测定的总数 核心不会减少，实验室人员现在更熟悉执行高容量分析，因此可以更有效地执行它们。不幸的是，这些检测试剂盒的供应价格并没有下降，因此我们无法像人员预算那样减少供应预算。