Core--Cell culture

细胞培养核心设施将提供设备（例如，工作站、储存设施和专用显微镜）和所有项目的材料技术支持。例如，它将维持我们全能干细胞的收集和房屋或大规模细胞培养设施，用于生产重组DNA衍生的和天然存在的蛋白质。该设施将为那些需要分离其他细胞产品的细胞器的项目产生大量细胞（例如，转录因子），并在细胞产物的分离/纯化中提供建议和物质帮助。它将作为一个中心的荧光（标准，倒置和共聚焦显微镜可用），并为大鼠和小鼠单克隆抗体的产生。它也将是天然和修饰的脂蛋白和脂蛋白缺乏血清，血清脂蛋白谱的FPLC分析，特殊培养基配方，介导测试和使用潜在致病试剂的设施的来源。该设施还将提供高灵敏度的Kodak Image Station 440 CF和1-D图像分析软件，用于免疫受体测定，其中可以很容易地测量与受体和转运蛋白结合的配体的定量。本项目所有个体均采用动物细胞培养技术。在某些情况下，培养的细胞代表主要的实验系统，而在其他情况下，这些细胞是用于产生动物模型的试剂或中间体的来源。细胞培养核心的目标是通过为该计划提供专门的设施和材料和技术支持，促进研究个人项目，并促进合作研究。

The Cell Culture Core facility will provide equipment (e.g., workstations, storage facilities and specialized microscopes) and material technical support for all of the projects. For example, it will maintain our collection of totipotent stem cells and houses or large scale cell culture facility for production of recombinant DNA-derived and naturally occurring proteins. The facility will generate large numbers of cells for those projects requiring the isolation of cellular organelles of other cell products (e.g., transcription factors), and provide advice and material assistance in the isolation/purification of the cell products. It will serve as a center for fluorescence (standard, inverted and confocal microscopes are available) and for the generation of both rat and mouse monoclonal antibodies. It will also be a source of native and modified lipoproteins and lipoprotein-deficient sera, FPLC analysis of serum lipoprotein profiles, special media formulations, mediating testing, and facilities for use of potentially pathogenic reagents. The facility will also provide a high sensitivity Kodak Image Station 440 CF and 1-D Image Analysis software for immunoreceptor assays, in which the quantitative of ligand binding to receptors and transporters can be readily measured. All of the individual of the Program employ animal cell culture techniques. In some cases of the cultured cells represent a primary experimental system, while in oth4ers these cells are sources of regents or of intermediates used for the generation of animal models. The goal of the Cell Culture Core is to facilitate research individual projects and to foster collaborative studies by providing specialized facilities and material and technical support to the Program.