Core--Cell culture

核心--细胞培养

• 批准号: 6869588
• 负责人: MONTY KRIEGER
• 金额: $ 21.64万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-12-10 至 2005-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6869588
• 关键词: biomedical facility; blood lipoprotein; fluorescence microscopy; monoclonal antibody; receptor binding; stem cells; tissue /cell culture

The Cell Culture Core facility will provide equipment (e.g., workstations, storage facilities and specialized microscopes) and material technical support for all of the projects. For example, it will maintain our collection of totipotent stem cells and houses or large scale cell culture facility for production of recombinant DNA-derived and naturally occurring proteins. The facility will generate large numbers of cells for those projects requiring the isolation of cellular organelles of other cell products (e.g., transcription factors), and provide advice and material assistance in the isolation/purification of the cell products. It will serve as a center for fluorescence (standard, inverted and confocal microscopes are available) and for the generation of both rat and mouse monoclonal antibodies. It will also be a source of native and modified lipoproteins and lipoprotein-deficient sera, FPLC analysis of serum lipoprotein profiles, special media formulations, mediating testing, and facilities for use of potentially pathogenic reagents. The facility will also provide a high sensitivity Kodak Image Station 440 CF and 1-D Image Analysis software for immunoreceptor assays, in which the quantitative of ligand binding to receptors and transporters can be readily measured. All of the individual of the Program employ animal cell culture techniques. In some cases of the cultured cells represent a primary experimental system, while in oth4ers these cells are sources of regents or of intermediates used for the generation of animal models. The goal of the Cell Culture Core is to facilitate research individual projects and to foster collaborative studies by providing specialized facilities and material and technical support to the Program.

细胞培养核心设施将为所有项目提供设备(如工作站、储存设施和专用显微镜)和材料技术支持。例如，它将维持我们收集的全能干细胞和房屋或大规模细胞培养设施，以生产重组DNA衍生和自然产生的蛋白质。该设施将为那些需要分离其他细胞产品(例如转录因子)的细胞器的项目产生大量细胞，并在细胞产品的分离/纯化方面提供建议和物质援助。它将作为荧光中心(有标准的倒置显微镜和共聚焦显微镜可用)，并用于产生大鼠和小鼠的单抗。它还将是天然和修饰的脂蛋白和脂蛋白缺乏血清的来源，血清脂蛋白谱的FPLC分析，特殊的介质配方，中介测试，以及使用潜在致病试剂的设施。该设施还将提供用于免疫受体分析的高灵敏度柯达图像站440 CF和1-D图像分析软件，在该软件中可以很容易地测量与受体和转运体结合的配体的数量。该计划的所有个人都采用了动物细胞培养技术。在某些情况下，培养的细胞代表一个主要的实验系统，而在另一些情况下，这些细胞是用于产生动物模型的试剂或中间体的来源。细胞培养核心的目标是通过为该计划提供专门的设施、材料和技术支持，促进单个项目的研究，并促进合作研究。