Core--Transgenic

核心--转基因

• 批准号: 7006139
• 负责人: MONTY KRIEGER
• 金额: $ 43.21万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-12-01 至 2005-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7006139
• 关键词: animal colony; biomedical facility; genetically modified animals; laboratory mouse

The application of transgenic and chimeric and approaches represent a major activity of this program. The Transgenic Core provides the scientific staff, mouse breeding colonies, and specialized equipment needed to generate transgenic mice as well as produce chimeric mice for the program's investigators. The services offered include generation of transgenic mice, production of embryonic stem cell chimeras, ovary transplantation, freezing of 8-cell embryos for preservation of mouse strains, embryo transfer to reconstitute mice contaminated with pathogens, maintenance of mouse lines, collection of embryos, and testing of ES cell lines for germ line transmission.

转基因和嵌合基因的应用是该项目的主要活动.转基因核心提供了科学工作人员，小鼠繁殖菌落，以及产生转基因小鼠所需的专门设备，以及为该计划的研究人员生产嵌合小鼠。所提供的服务包括转基因小鼠的产生、胚胎干细胞嵌合体的生产、卵巢移植、冷冻8细胞胚胎以保存小鼠品系、胚胎移植以重建受病原体污染的小鼠、维持小鼠品系、收集胚胎以及测试胚胎干细胞系以进行生殖系传播。