Core--Transgenic

核心--转基因

• 批准号: 7006139
• 负责人: MONTY KRIEGER
• 金额: $ 43.21万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-12-01 至 2005-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7006139
• 关键词: animal colony; biomedical facility; genetically modified animals; laboratory mouse

The application of transgenic and chimeric and approaches represent a major activity of this program. The Transgenic Core provides the scientific staff, mouse breeding colonies, and specialized equipment needed to generate transgenic mice as well as produce chimeric mice for the program's investigators. The services offered include generation of transgenic mice, production of embryonic stem cell chimeras, ovary transplantation, freezing of 8-cell embryos for preservation of mouse strains, embryo transfer to reconstitute mice contaminated with pathogens, maintenance of mouse lines, collection of embryos, and testing of ES cell lines for germ line transmission.

转基因和嵌合和方法的应用代表了该程序的主要活动。转基因核心为产生转基因小鼠所需的科学人员，小鼠育种菌落以及为该计划的研究人员生产嵌合小鼠所需的专门设备。提供的服务包括产生转基因小鼠，产生胚胎干细胞嵌合体，卵巢移植，冻结8个胚胎以保存小鼠菌株，胚胎转移以污染病原体污染的小鼠，维持被小鼠线，小鼠系列的收集和ES细胞线的胚胎线条传输的测试。