Flavivirus replication - biogenesis, ultrastructure and roles of induced membranes

黄病毒复制 - 生物发生、超微结构和诱导膜的作用

• 批准号: nhmrc : 102481
• 负责人: A/Pr Jason Mackenzie
• 金额: $ 22.33万
• 依托单位国家: 澳大利亚
• 项目类别: NHMRC Project Grants
• 财政年份: 2001
• 资助国家: 澳大利亚
• 起止时间: 2001-01-01 至 2002-12-31
• 项目状态: 已结题
• 来源: https://researchdata.edu.au/flavivirus-replication-biogenesis-induced-membranes/94856
• 关键词: Flavivirus; replication; biogenesis; ultrastructure; roles

Flaviviruses are the agents of many mosquito-transmitted infections such as encephalitis and dengue, and hepatitis C virus is a member of the same virus family. During virus multiplication in cells, new membrane structures are induced and these represent regions where the replication events occur. Using Kunjin virus, an agent of Australian encephalitis, as a model, and advanced techniques in biochemistry and electron microscopy, we have previously identified these membranes as the site of synthesis of the viral RNA or genetic material, and the viral proteins involved. These comprise the viral replication complex. The research will define the origin of these membranes, and how the components of the associated replication complex are assembled. Assembly of the virus particles in cells is also being analysed using similar technology. Hepatitis C virus cannot be reliably grown at present for research purposes in cultured cells, and we will attempt to develop a helper system to overcome the problem. An understanding of these processes, and how the viral RNA is copied into progeny RNA for new virus particles, may assist in the development of antiviral drugs for treatment of slow or persistent virus infections such as hepatitis C.

黄病毒是许多蚊子传播的感染（例如脑炎和登革热）的病原体，丙型肝炎病毒是同一病毒家族的成员。病毒在细胞中增殖期间，会诱导新的膜结构，这些结构代表复制事件发生的区域。使用昆金病毒（澳大利亚脑炎的一种病原体）作为模型，以及先进的生物化学和电子显微镜技术，我们之前已经确定这些膜是病毒RNA或遗传物质以及相关病毒蛋白的合成位点。它们组成了病毒复制复合体。该研究将确定这些膜的起源，以及相关复制复合物的组成部分是如何组装的。细胞中病毒颗粒的组装也正在使用类似的技术进行分析。目前丙型肝炎病毒无法在培养细胞中可靠地生长以用于研究目的，我们将尝试开发辅助系统来克服该问题。了解这些过程，以及病毒 RNA 如何复制到新病毒颗粒的后代 RNA 中，可能有助于开发用于治疗慢性或持续性病毒感染（如丙型肝炎）的抗病毒药物。