MECHANICAL ENVIRONMENT IN MANDIBULAR DISTRACTION

下颌牵引的机械环境

• 批准号: 7002332
• 负责人: SUSAN W HERRING
• 金额: $ 22.21万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7002332
• 关键词: biomechanics; cardiovascular system; gene expression; immunocytochemistry; juvenile animal; mandible /maxilla; mandibular condyle; mastication; miniature swine; muscle function; muscle proteins; osteogenesis; osteogenesis imperfecta; periosteums; radiography; stainings; temporomandibular joint syndrome; tissue /cell culture; ultrasonography

DESCRIPTION (provided by applicant): Distraction osteogenesis (DO) is a means to elongate bone by placing tensile loads on a healing osteotomy. Although DO has been used on the mandible for only a decade, the technique is already supplanting more traditional orthognathic procedures, especially for children. Despite the fact that DO uses mechanics to accomplish its goals, the actual mechanical environment is unknown. Functions such as mastication must place intermittent compressive or shearing strains on the distraction site. These strains, as well as the tensile strains produced by the distraction appliance, are of critical biological significance for the osteogenic process, but have never been measured. Using a well-established model for mandibular DO, the pig, we propose (Aim 1) to clarify the mechanical environment of the distraction site at the time of osteotomy, during distraction, and during consolidation by making direct measurements of strain using strain gages, digital ultrasonics and differential variable reluctance transducers. These studies will investigate the influence of appliance size, stability, and placement as well as the action of specific muscles and occlusal loads. Further, we will address a major concern of treating growing children by mandibular DO, whether the procedure adversely affects growth at the mandibular condylar by overloading the TMJ. Thus, we propose (Aim 2) to ascertain the impact of DO on TMJ mechanics and growth by performing strain gage measurements on the condylar neck and relating the findings to growth, as measured by mineral apposition rate and periosteal deformation. The mechanical environment of DO, particularly the stability of the distraction site, may affect the course of healing and the final result by influencing which cells are recruited, the rapidity with which they proliferate, and their eventual fate. Therefore, we propose (Aim 3) to relate the mechanical history of DO to the repair process by assessing the contributions of the periosteum, muscle connective tissue, and vascular system to the regenerate. This will be accomplished by pulse labeling of replicating cells during distraction, combined with an investigation of protein expression at the time of sacrifice. The most important contributions of this proposal will be the characterization of strain at the DO site under natural conditions, and the application of that information to understanding of how DO works on a cellular level and how it affects mandibular growth.

描述（由申请人提供）：牵引成骨（DO）是一种手段 通过在愈合截骨上施加拉力来拉长骨骼。虽然做 该技术在下颌骨上的应用仅十年时间，但已被广泛应用 取代更传统的正颌手术，尤其是对于儿童。 尽管 DO 使用机制来实现其目标，但实际情况 机械环境未知。咀嚼等功能必须放置 牵引部位间歇性的压缩或剪切应变。这些 应变，以及牵引器具产生的拉伸应变， 对于成骨过程具有重要的生物学意义，但 从未被测量过。使用成熟的猪下颌 DO 模型， 我们建议（目标 1）澄清分心的机械环境 截骨时、牵引时和巩固时的部位 使用应变计、数字超声波直接测量应变 和差动可变磁阻传感器。这些研究将 还研究设备尺寸、稳定性和放置的影响 作为特定肌肉和咬合负荷的动作。此外，我们将解决 通过下颌 DO 治疗成长中的儿童的一个主要问题是，是否 手术会因超载而对下颌髁突的生长产生不利影响 颞下颌关节。因此，我们建议（目标 2）确定 DO 对 TMJ 的影响 通过对髁突进行应变计测量来实现力学和生长 颈部并将结果与​​生长联系起来，通过矿物质并置测量 率和骨膜变形。 DO 的机械环境，特别是 分心部位的稳定性可能会影响愈合过程和 通过影响哪些细胞被招募、招募的速度来影响最终结果 它们增殖，以及它们最终的命运。因此，我们建议（目标 3） 通过评估将 DO 的机械历史与修复过程联系起来 骨膜、肌肉结缔组织和血管系统的贡献 到重生。这将通过复制的脉冲标记来完成 分散注意力期间的细胞，结合蛋白质表达的研究 在牺牲的时候。该提案最重要的贡献 将是自然条件下 DO 位点应变的表征， 以及应用该信息来理解 DO 如何在 细胞水平及其如何影响下颌生长。

项目成果

Mandibular mechanics after osteotomy and distraction appliance placement I: Postoperative mobility of the osteotomy site.

截骨和牵引矫治器放置后的下颌力学 I：截骨部位的术后活动度。

• DOI: 10.1016/j.joms.2005.12.008
• 发表时间: 2006
• 期刊: Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons
• 作者: Sun,Zongyang;Rafferty,KatherineL;Egbert,MarkA;Herring,SusanW
• 通讯作者: Herring,SusanW

The effect of periosteal injury and masticatory micromovement on the healing of a mandibular distraction osteogenesis site.

• DOI: 10.1016/j.archoralbio.2008.12.002
• 发表时间: 2009-03
• 期刊: ARCHIVES OF ORAL BIOLOGY
• 影响因子: 3
• 作者: Sun, Zongyang;Herring, Susan W.
• 通讯作者: Herring, Susan W.

Changes in growth and morphology of the condyle following mandibular distraction in minipigs: Overloading or underloading?

• DOI: 10.1016/j.archoralbio.2007.04.014
• 发表时间: 2007-10-01
• 期刊: ARCHIVES OF ORAL BIOLOGY
• 影响因子: 3
• 作者: Rafferty, Katherine L.;Sun, Zongyang;Herring, Susan W.
• 通讯作者: Herring, Susan W.