Histology and Stereology Support Core

组织学和体视学支持核心的建立是为了最大限度地利用现有资源， 小鼠脑组织处理，石蜡或冷冻包埋，连续切片和组织学染色， 项目1、2、3和4的体视学和合作研究部分。核心B还提供 处理、包埋、切片和组织学检查所需的设备、用品和专业知识 通过主任和临床合作者确定患者的脑组织染色。的目标 核心B包括： 1.成年和生后脑组织的制备及体视学方法的标准化 遗传小鼠模型对于PPG中的所有型号，核心将产生有助于直接 使用高度标准化的组织学和定量解剖学方法比较小鼠品系。 2.模型的全脑表型。大脑将在出生后的关键年龄进行石蜡包埋， 用组织化学（H & E/NissI）和免疫学染色进行表型分型。数据将包括主要 细胞核;总细胞数和病理细胞谱。将评估投射神经元代表性 通过细胞形态学和免疫组织化学（IHC）。 3.行为特征小鼠的大脑。在行为上投入的时间和资源 测试使得从行为研究中获得的所有大脑的石蜡包埋和存档具有成本效益。这些 组织可用于确认同一队列中的解剖学效应或检验posf hoc假设 与已知的行为表型有关。 4.在一系列胎龄的多基因型胚胎（一些BrdU脉冲标记）。 项目1、2和3实验产生的额外胚胎将被处理，石蜡包埋， 存档以备以后随着工作的进展对新问题和假设进行调查时使用。 5.相关的人类大脑病理学。虽然啮齿动物模型表明，人类疾病影响 可能主要是中间神经元，但人类数据大多缺乏。核心B将处理组织块或固定 通过主任和临床合作者确定的组织。制备用于组织化学的载玻片， 核心B的IHC将由项目1和2的研究者进行分析。 6.小鼠库存和识别。虽然每个项目将维护自己的鼠标线，核心B， 将定期更新通过PPG提供的遗传模型的命名和库存， 促进各项目之间的协调，以便采购和促进对有用线路的初步分析， 十字架

The histology and stereology support core was created to maximize the use of available resources for mouse brain tissue processing, paraffin or cryo- embedding, serial sectioning and histological staining of sections for stereology and collaborative studies involving Projects 1, 2, 3 and 4. Core B also provides equipment, supplies and expertise required for the processing, embedding, sectioning and histological staining of brain tissue from patients ascertained through the Director and clinical collaborators. The goals of Core B include: 1. Preparation of tissue and standardization of stereological methods in adult and postnatal brain of genetic mouse models. For all models in the PPG the core will produce material facilitating the direct comparison of mouse strains using highly standardized histological and quantitative anatomical methods. 2. Whole-brain phenotyping of models. Brains will be paraffin-embedded at key postnatal ages for phenotyping with histochemical (H&E/NissI) and immunological stains. Data will include sizes of major nuclei; total cell number and pathological cell profiles. Projection neuron representation will be assessed by cell morphology and immunohistochemistry (IHC). 3. Archiving brains of behaviorally characterized mice. The time and resources invested in behavioral testing makes it cost effective to paraffin embed and archive all brains from behavioral studies. These tissues can be used to confirm an anatomical effect in the same cohort or test posf hoc hypotheses related to known behavioral phenotypes. 4. Archiving embryos (some BrdU pulse-labeled) of multiple genotypes at a range of gestational ages. Extra embryos generated by Projects 1,2 & 3 experiments will be processed, paraffin embedded and archived to be available for later investigation of new questions and hypotheses as the work progresses. 5. Correlative human brain pathology. While rodent models suggest that human disorders affecting primarily interneurons are likely, human data are mostly lacking. Core B will process tissue blocks or fixed tissues ascertained through the Director and clinical collaborators. Slides prepared for histochemistry and IHC in Core B will be analyzed by Project 1 and 2 investigators. 6. Mouse inventory and identification. Although each project will maintain its own mouse lines, Core B, will periodically update the nomenclature and inventory of genetic models available through the PPG and facilitate coordination across projects in order to procure and facilitate initial analysis of useful lines and crosses.