Fluorescence Enhanced Photothermal Infrared Spectroscopy (FE-PTIR) - breakthrough for simultaneous fluorescence microscopy and sub-micron IR spectroscopy
荧光增强光热红外光谱 (FE-PTIR) - 同步荧光显微镜和亚微米红外光谱的突破
基本信息
- 批准号:10693270
- 负责人:
- 金额:$ 86.15万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2021
- 资助国家:美国
- 起止时间:2021-04-02 至 2024-08-31
- 项目状态:已结题
- 来源:
- 关键词:Alzheimer&aposs DiseaseAmyloidAntimicrobial ResistanceAntimicrobial susceptibilityAreaBiochemicalBiocompatible MaterialsBiologicalBiological SciencesBiologyBiomedical EngineeringBiomedical ResearchCellsChemical StructureChemicalsClassificationComputer softwareConfocal MicroscopyDataData AnalysesDependenceDetectionDevelopmentDisease ResistanceFluorescenceFluorescence MicroscopyGlucoseGoalsHeatingHigh temperature of physical objectImageIn SituIndividualInfrared RaysIsotopesLabelLasersLightLipidsMalignant NeoplasmsMapsMeasurementMeasuresMetabolismMicroscopeMolecularMolecular AnalysisMolecular StructureMultimodal ImagingNeurodegenerative DisordersNeuronsOptical InstrumentPharmaceutical PreparationsPharmacotherapyPhasePhotobleachingPhysiologic pulsePositioning AttributeProtein AnalysisProteinsPublicationsResearchResolutionSamplingSmall Business Innovation Research GrantSourceSpecimenSpectrum AnalysisStructureTechniquesTechnologyTemperatureTestingTissuesUnited States National Institutes of HealthVisible Radiationabsorptionantimicrobialbiological researchbiomedical imagingbrain tissuecancer cellcommercializationdata acquisitiondesignengineering designfluorescence imagingfluorophoreglucose metabolismimprovedinfrared microscopyinfrared spectroscopyinnovationinsightinterestmetermicrobialmultimodalitynoveloptical imagingprotein foldingprotein misfoldingprototyperesponsespectroscopic imagingsubmicrontau Proteinsvibration
项目摘要
1 This Phase II project aims to develop and commercialize Fluorescence Enhanced Photothermal Infrared (FE-PTIR) imaging
2 and spectroscopy. The proposed FE-PTIR will use fluorescence microscopy to map the distribution of fluorescently labeled
3 regions of cells and tissue and then provide chemical structural analysis of the labeled regions using photothermal infrared
4 spectroscopy. Fluorescence microscopy is a cornerstone technique in biological research, allowing sensitive mapping of
5 specifically targeted biomolecules within cells and tissue, but it does not provide information about their molecular
6 structure. Infrared (IR) spectroscopy can provide rich analysis of molecular structure and has been used in life sciences
7 research to study tissue classification, drug/tissue interaction, neurodegenerative diseases, cancer and other areas.
8 Conventional IR spectroscopy, however, has a fundamental spatial resolution limit (i.e. roughly how small an object it can
9 analyze) of around 10 micrometers, similar to the size of an average biological cell. Thus conventional IR spectroscopy has
10 been extremely limited for many biomedical applications where the structures of interest are smaller than a cell.
11 The FE-PTIR technique illuminates a fluorescently labeled sample with UV/visible light which results in fluorescent
12 emission from fluorescently tagged molecules in the sample. A tunable infrared laser source also illuminates the sample,
13 causing localized heating in the sample if the IR laser is tuned to a wavelength that excites molecular vibrations in the
14 sample. Using a camera or other sensitive photodetector is used to record the fluorescent emission from different regions
15 of the sample generates a map of the distribution of fluorescently labeled biomolecules. A key innovation of this proposal
16 was the recognition that common fluorophores have an emission efficiency that is highly temperature dependent. Thus
17 when the sample is also irradiated with infrared light at wavelengths corresponding to molecular vibrations, localized
18 heating from IR absorption by the sample causes a significant change in the fluorescent emission. Recording the emission
19 change as a function of sample position or IR wavelength produces IR absorption images and IR absorption spectra,
20 respectively. Phase I research demonstrated the following key advances: (1) ability of FE-PTIR to map of target
21 biomolecules with fluorescence and analyze the molecular structural of the target molecules; (2) achieve submicron
22 spatial resolution for both fluorescence imaging and infrared spectroscopy; (3) demonstrated a 100X improvement in
23 measurement sensitivity; (4) application of FE-PTIR to study of protein misfolding relevant to neurodegenerative disease
24 research; (5) demonstrated FE-PTIR on individual bacterial and live cancer cells with subcellular resolution. This project is
25 well aligned with NIH goals as it incorporates several key thrusts of the National Institute of Biomedical Imaging and
26 Bioengineering, including optical imaging and spectroscopy, IR imaging, confocal microscopy, and multimodal imaging. FE-
27 PTIR will be extremely useful for example in analyzing the molecular structure/folding/aggregation of fluorescence-
28 localized proteins. Protein misfolding/aggregation is a root cause of many neurodegenerative diseases (e.g. Alzheimer’s).
29 Completion of this Phase II project will lead to the commercialization of a new multimodal microscope that will offer
30 profound benefits for biomedical research including neurodegenerative diseases and antimicrobial resistance research.
本二期项目旨在开发和商业化荧光增强光热红外(FE-PTIR)成像技术
项目成果
期刊论文数量(3)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Optical Photothermal Infrared Microspectroscopy with Simultaneous Raman - A New Non-Contact Failure Analysis Technique for Identification of <10 μm Organic Contamination in the Hard Drive and other Electronics Industries.
- DOI:10.1017/s1551929520000917
- 发表时间:2020-05
- 期刊:
- 影响因子:0
- 作者:Kansiz M;Prater C;Dillon E;Lo M;Anderson J;Marcott C;Demissie A;Chen Y;Kunkel G
- 通讯作者:Kunkel G
Analysis of Fixed and Live Single Cells Using Optical Photothermal Infrared with Concomitant Raman Spectroscopy.
- DOI:10.1021/acs.analchem.0c04846
- 发表时间:2021-03-02
- 期刊:
- 影响因子:7.4
- 作者:Spadea A;Denbigh J;Lawrence MJ;Kansiz M;Gardner P
- 通讯作者:Gardner P
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Craig Prater其他文献
Craig Prater的其他文献
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{{ truncateString('Craig Prater', 18)}}的其他基金
Fluorescence Enhanced Photothermal Infrared Spectroscopy (FE-PTIR) - breakthrough for simultaneous fluorescence microscopy and sub-micron IR spectroscopy
荧光增强光热红外光谱 (FE-PTIR) - 同步荧光显微镜和亚微米红外光谱的突破
- 批准号:
10543927 - 财政年份:2021
- 资助金额:
$ 86.15万 - 项目类别:
Fluorescence Enhanced Photothermal Infrared Spectroscopy (FE-PTIR)-breakthrough for simultaneous fluorescence microscopy and sub-micron IR spectroscopy
荧光增强光热红外光谱 (FE-PTIR)——同步荧光显微镜和亚微米红外光谱的突破
- 批准号:
10253663 - 财政年份:2021
- 资助金额:
$ 86.15万 - 项目类别:
IRaman: Breakthrough biomedical microscope with simultaneous infrared and Raman spectroscopy at sub-micron spatial resolution
IRaman:具有亚微米空间分辨率同时红外和拉曼光谱的突破性生物医学显微镜
- 批准号:
10006670 - 财政年份:2018
- 资助金额:
$ 86.15万 - 项目类别:
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