Location location location - how genome position affects gene expression

细菌基因组已经进化了数十亿年，以便在正确的时间有效表达正确的基因。很明显，同一个基因，放在细菌染色体周围的不同位置，可以显示出非常不同的表达水平。然而，管理这一点的规则尚未完全理解。该项目旨在研究基因组中的位置如何影响基因表达，这将有助于我们了解基因组如何工作和进化，并使我们能够合理设计合成染色体，从而优化重要基因的表达。该项目将比较沙门氏菌和大肠杆菌中数百万份相同基因启动子的表达。大肠杆菌-两个相关的物种，共享一些基因组组织，但控制基因表达不同-使用工具，使表达在不同的条件下进行测量，并混合分子微生物学，转录组学和信息学。

Bacterial genomes have evolved over billions of year to allow efficient expression of the right genes at the right times. It has become clear that the same gene, placed in different locations around a bacterial chromosome can display very different levels of expression. The rules which govern this are however not fully understood. This project aims to examine how position within a genome can affect gene expression, this will help us both understand how genomes work and evolve, and allow us to rationally design synthetic chromosomes which will deliver optimised expression of important genes. The project will compare expression of millions of copies of the same gene promoters in both Salmonella and E. coli - two related species which share some genome organisation but control gene expression differently - using tools to allow expression to be measured under different conditions, and a mix of molecular microbiology, transcriptomics and informatics.