Location location location - how genome position affects gene expression

Location location 位置 - 基因组位置如何影响基因表达

• 批准号: 2748815
• 金额: --
• 依托单位: University of East Anglia
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2022
• 资助国家: 英国
• 起止时间: 2022 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2748815
• 关键词: Location; location; how; genome; position

Bacterial genomes have evolved over billions of year to allow efficient expression of the right genes at the right times. It has become clear that the same gene, placed in different locations around a bacterial chromosome can display very different levels of expression. The rules which govern this are however not fully understood. This project aims to examine how position within a genome can affect gene expression, this will help us both understand how genomes work and evolve, and allow us to rationally design synthetic chromosomes which will deliver optimised expression of important genes. The project will compare expression of millions of copies of the same gene promoters in both Salmonella and E. coli - two related species which share some genome organisation but control gene expression differently - using tools to allow expression to be measured under different conditions, and a mix of molecular microbiology, transcriptomics and informatics.

细菌基因组经过数十亿年的进化，能够在正确的时间高效地表达正确的基因。现在已经很清楚，同一个基因，如果放置在细菌染色体周围的不同位置，会显示出非常不同的表达水平。然而，控制这种情况的规则还没有完全被理解。该项目旨在研究基因组内的位置如何影响基因表达，这将帮助我们了解基因组如何工作和进化，并允许我们合理设计合成染色体，这将提供重要基因的优化表达。该项目将比较沙门氏菌和大肠杆菌中相同基因启动子的数百万个拷贝的表达——这是两个相关的物种，它们共享一些基因组组织，但控制基因表达的方式不同——使用允许在不同条件下测量表达的工具，以及分子微生物学、转录组学和信息学的混合。