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Crossing paths: Investigating the role of auxin and cytokinin in 3D growth specification

交叉路径：研究生长素和细胞分裂素在 3D 生长规范中的作用

基本信息

批准号：
2748819
负责人：
金额：
--
依托单位：
University of Oxford
依托单位国家：
英国
项目类别：
Studentship
财政年份：
2022
资助国家：
英国
起止时间：
2022 至无数据
项目状态：
未结题

来源：
https://gtr.ukri.org/projects?ref=studentship-2748819
关键词：
Crossing paths Investigating role auxin

项目摘要

One of the most important events in history was the colonization of land by plants approximately 470 million years ago. This transition coincided with, and was likely facilitated by, the evolution of 3-dimensional (3D) growth. 3D growth is an invariable and pivotal feature of all land plants, and the diverse morphologies exhibited across the globe are a result of the differential regulation of 3D growth processes. Yet, we know very little about how 3D growth is regulated at the genetic level.Studies of 3D growth are difficult using flowering plants as 3D growth begins within the first few divisions of the zygote. Disrupting 3D growth in these plants would therefore cause embryo lethality and provide a narrow timeframe for experimental investigation. Conversely, in the moss Physcomitrium patens, 3D growth (gametophore development) is preceded by a protracted 2-dimensional (2D) filamentous phase that can be cultured indefinitely. P. patens does not need to initiate 3D growth to survive, and thus is an ideal model system to genetically dissect 3D growth. The 2D to 3D growth transition in P. patens is dependent on auxin and cytokinin signaling, is accompanied by highly coordinated changes in cell division plane orientation and culminates in the formation of gametophores with radially organised leaf-like phyllids. Auxin has been consistently implicated in cell division plane orientation within a broad range of developmental contexts and has been implicated as the 'instructive signal' for division plane orientation in developing gametophores.We have isolated a number of 'no gametophores' (nog) mutants that fail to specify 3D growth because they exhibit defects in cell division orientation. This proposal will exploit these 'nog' mutants to unravel the complex auxin-cytokinin crosstalk underpinning the 2D to 3D growth transition in P. patens and will provide unprecedented insight into the regulation of 3D growth in plants.Project Objectives:-Identify the mutations in no gametophore' (nog) mutants that fail to specify 3D growth- Characterize the auxin and cytokinin responses in nog mutants and obtain transcriptomes-Recapitulate 3D growth defects through cell-specific disruption of auxin/cytokinin signaling

历史上最重要的事件之一是大约4.7亿年前植物对土地的殖民化。这种转变与三维（3D）生长的演变相吻合，并可能受到其促进。3D生长是所有陆地植物不变的关键特征，并且在地球仪上表现出的不同形态是3D生长过程的差异调节的结果。然而，我们对3D生长在遗传水平上是如何调节的知之甚少。3D生长的研究很难使用开花植物，因为3D生长开始于受精卵的前几次分裂。因此，破坏这些植物的3D生长将导致胚胎死亡，并为实验研究提供狭窄的时间范围。相反，在藓类立碗藓中，3D生长（配子体发育）之前是可以无限期培养的延长的二维（2D）丝状阶段。展叶侧耳不需要启动三维生长就能存活，因此是一种理想的三维生长遗传解剖模型系统。P. patens的2D到3D生长转变依赖于生长素和细胞分裂素信号传导，伴随着细胞分裂平面方向的高度协调变化，并最终形成具有放射状组织的叶状体的配子体。生长素一直牵连在细胞分裂平面的方向在广泛的发展背景下，并已牵连作为'指导性信号'为分裂平面的方向在发展gametophores.We已经分离出一些'没有gametophores'（nog）的突变体，未能指定3D生长，因为他们表现出缺陷的细胞分裂方向。该计划将利用这些“nog”突变体来解开复杂的生长素-细胞分裂素串扰，这是P. patens从2D向3D生长转变的基础，并将为植物3D生长的调控提供前所未有的见解。项目目标：-鉴定无配子体（nog）突变体中不能指定3D生长的突变-表征nog突变体中生长素和细胞分裂素的响应并获得转录组-通过细胞特异性破坏生长素/细胞分裂素信号转导来概括3D生长缺陷