Fluorescence imaging flow cytometry in non-straight microfluidic channels
非直微流体通道中的荧光成像流式细胞术
基本信息
- 批准号:2749998
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:英国
- 项目类别:Studentship
- 财政年份:2022
- 资助国家:英国
- 起止时间:2022 至 无数据
- 项目状态:未结题
- 来源:
- 关键词:
项目摘要
Cells can be manipulated using forces generated by flow, either to probe their mechanical properties or to deliver cargo (e.g. drugs) into the cells. This can be performed in special microfluidic devices. A limitation of these devices, due to the high flow rates and complex geometry, is that it is currently impossible to take fluorescence images of the cells as they flow through. These images are essential for understanding cell behaviour under these extreme conditions, as fluorescence can report on the response of various cellular components (cytoskeleton, membrane, mitochondria) to stress. The aim of this project is to implement a high-speed fluorescence light sheet microscope to view cells under high shear stress in a non-straight microfluidic platform. This will be used to improve our understanding of cellular responses to shear deformation, which has potential application of identifying cancerous cells as well as delivering probes and drugs into cells. In order to create a shear stress, a flow cytometer will be manufactured in a cross-flow junction geometry, where fluid flows in and exits through two separate channels and the cells experience large shear stresses at a stationary central point. This geometry however makes implementing a fluorescence microscope challenging. Oblique plane microscopy will be used, as this technology is able to apply light sheet microscopy in an inverted geometry (viewing the microfluidic device from the bottom). Meanwhile the light-sheet is essential to minimise out-of-focus fluorescence to generate high-contrast images at high speeds. With this setup the aim is to achieve imaging at 200,000 frames-per-second, using an intensified high-speed camera. This will in turn allow measurements of 100,000 cells/min with the cells flowing at speeds of up to 1 m/s in the flow cytometer. When cells experience high shear stresses they respond by undergoing substantial stretching. This stretching has been shown to depend on the type of cell, such that cross-flow cytometry can be used to identify cancerous cells. However, little is known about which cellular components are responsible for deformation under these conditions and this is where the new cytometer will be applied to study how various cell component control the deformation. Furthermore, as cells are stretched pores are formed in the cell membrane to account for the increased surface area. This offers potential opportunities for drug delivery or the transport of small molecules into cells due to these pores being created during deformation. With the addition of fluorescence microscopy, our understanding of the cellular mechanisms responsible for the creation of these pores will be improved. With an increased understanding of pore formation (e.g. pore size distribution, lifetime, stability), there is a possibility to model and describe their capability of acting as transport vehicles for intracellular delivery.
可以使用流动产生的力来操纵细胞,以探测它们的机械特性或将货物(例如药物)递送到细胞中。这可以在特殊的微流体装置中进行。由于高流速和复杂的几何形状,这些装置的局限性在于,目前不可能在细胞流过时拍摄细胞的荧光图像。这些图像对于了解这些极端条件下的细胞行为至关重要,因为荧光可以报告各种细胞成分(细胞骨架,膜,线粒体)对压力的反应。本计画的目的是实现一种高速萤光片层显微镜,以观察在高剪切应力下的非直线微流控平台中的细胞。这将用于提高我们对剪切变形的细胞反应的理解,这具有识别癌细胞以及将探针和药物递送到细胞中的潜在应用。为了产生剪切应力,流式细胞仪将被制造成交叉流连接几何形状,其中流体通过两个单独的通道流入和流出,并且细胞在固定的中心点处经历大的剪切应力。然而,这种几何形状使得实现荧光显微镜具有挑战性。将使用斜平面显微镜,因为该技术能够以倒置几何形状(从底部观察微流体装置)应用光片显微镜。同时,光片对于最大限度地减少散焦荧光以高速生成高对比度图像至关重要。通过这种设置,目标是使用增强型高速相机实现每秒200,000帧的成像。这又将允许在流式细胞仪中以高达lm/s的速度流动的细胞的情况下测量100,000个细胞/min。当细胞经历高剪切应力时,它们通过经历大量拉伸来响应。这种拉伸已被证明取决于细胞的类型,因此交叉流式细胞术可用于识别癌细胞。然而,很少有人知道在这些条件下哪些细胞成分负责变形,这就是新的细胞仪将被应用于研究各种细胞成分如何控制变形的地方。此外,当细胞被拉伸时,细胞膜中形成孔以解释增加的表面积。这为药物递送或将小分子运输到细胞中提供了潜在的机会,因为这些孔在变形期间产生。随着荧光显微镜的加入,我们对这些孔的产生的细胞机制的理解将得到改善。随着对孔形成(例如孔径分布、寿命、稳定性)的理解的增加,有可能对它们作为细胞内递送的运输媒介物的能力进行建模和描述。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
其他文献
Internet-administered, low-intensity cognitive behavioral therapy for parents of children treated for cancer: A feasibility trial (ENGAGE).
针对癌症儿童父母的互联网管理、低强度认知行为疗法:可行性试验 (ENGAGE)。
- DOI:
10.1002/cam4.5377 - 发表时间:
2023-03 - 期刊:
- 影响因子:4
- 作者:
- 通讯作者:
Differences in child and adolescent exposure to unhealthy food and beverage advertising on television in a self-regulatory environment.
在自我监管的环境中,儿童和青少年在电视上接触不健康食品和饮料广告的情况存在差异。
- DOI:
10.1186/s12889-023-15027-w - 发表时间:
2023-03-23 - 期刊:
- 影响因子:4.5
- 作者:
- 通讯作者:
The association between rheumatoid arthritis and reduced estimated cardiorespiratory fitness is mediated by physical symptoms and negative emotions: a cross-sectional study.
类风湿性关节炎与估计心肺健康降低之间的关联是由身体症状和负面情绪介导的:一项横断面研究。
- DOI:
10.1007/s10067-023-06584-x - 发表时间:
2023-07 - 期刊:
- 影响因子:3.4
- 作者:
- 通讯作者:
ElasticBLAST: accelerating sequence search via cloud computing.
ElasticBLAST:通过云计算加速序列搜索。
- DOI:
10.1186/s12859-023-05245-9 - 发表时间:
2023-03-26 - 期刊:
- 影响因子:3
- 作者:
- 通讯作者:
Amplified EQCM-D detection of extracellular vesicles using 2D gold nanostructured arrays fabricated by block copolymer self-assembly.
使用通过嵌段共聚物自组装制造的 2D 金纳米结构阵列放大 EQCM-D 检测细胞外囊泡。
- DOI:
10.1039/d2nh00424k - 发表时间:
2023-03-27 - 期刊:
- 影响因子:9.7
- 作者:
- 通讯作者:
的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('', 18)}}的其他基金
An implantable biosensor microsystem for real-time measurement of circulating biomarkers
用于实时测量循环生物标志物的植入式生物传感器微系统
- 批准号:
2901954 - 财政年份:2028
- 资助金额:
-- - 项目类别:
Studentship
Exploiting the polysaccharide breakdown capacity of the human gut microbiome to develop environmentally sustainable dishwashing solutions
利用人类肠道微生物群的多糖分解能力来开发环境可持续的洗碗解决方案
- 批准号:
2896097 - 财政年份:2027
- 资助金额:
-- - 项目类别:
Studentship
A Robot that Swims Through Granular Materials
可以在颗粒材料中游动的机器人
- 批准号:
2780268 - 财政年份:2027
- 资助金额:
-- - 项目类别:
Studentship
Likelihood and impact of severe space weather events on the resilience of nuclear power and safeguards monitoring.
严重空间天气事件对核电和保障监督的恢复力的可能性和影响。
- 批准号:
2908918 - 财政年份:2027
- 资助金额:
-- - 项目类别:
Studentship
Proton, alpha and gamma irradiation assisted stress corrosion cracking: understanding the fuel-stainless steel interface
质子、α 和 γ 辐照辅助应力腐蚀开裂:了解燃料-不锈钢界面
- 批准号:
2908693 - 财政年份:2027
- 资助金额:
-- - 项目类别:
Studentship
Field Assisted Sintering of Nuclear Fuel Simulants
核燃料模拟物的现场辅助烧结
- 批准号:
2908917 - 财政年份:2027
- 资助金额:
-- - 项目类别:
Studentship
Assessment of new fatigue capable titanium alloys for aerospace applications
评估用于航空航天应用的新型抗疲劳钛合金
- 批准号:
2879438 - 财政年份:2027
- 资助金额:
-- - 项目类别:
Studentship
Developing a 3D printed skin model using a Dextran - Collagen hydrogel to analyse the cellular and epigenetic effects of interleukin-17 inhibitors in
使用右旋糖酐-胶原蛋白水凝胶开发 3D 打印皮肤模型,以分析白细胞介素 17 抑制剂的细胞和表观遗传效应
- 批准号:
2890513 - 财政年份:2027
- 资助金额:
-- - 项目类别:
Studentship
Understanding the interplay between the gut microbiome, behavior and urbanisation in wild birds
了解野生鸟类肠道微生物组、行为和城市化之间的相互作用
- 批准号:
2876993 - 财政年份:2027
- 资助金额:
-- - 项目类别:
Studentship
相似国自然基金
PET/MR多模态分子影像在阿尔茨海默病炎症机制中的研究
- 批准号:82372073
- 批准年份:2023
- 资助金额:48.00 万元
- 项目类别:面上项目
用于小尺寸管道高分辨成像荧光聚合物点的构建、成像机制及应用研究
- 批准号:82372015
- 批准年份:2023
- 资助金额:48.00 万元
- 项目类别:面上项目
基于影像代谢重塑可视化的延胡索酸水合酶缺陷型肾癌危险性分层模型的研究
- 批准号:82371912
- 批准年份:2023
- 资助金额:48.00 万元
- 项目类别:面上项目
发展双模态超分辨率全景成像技术,描绘自噬和迁移性胞吐过程中的细胞器互作网络
- 批准号:92054301
- 批准年份:2020
- 资助金额:900.0 万元
- 项目类别:重大研究计划
活细胞单分子成像定量研究EGFR内吞途径命运选择
- 批准号:32000557
- 批准年份:2020
- 资助金额:24.0 万元
- 项目类别:青年科学基金项目
基于多尺度三维重构与拓扑分析的种子休眠与发育调控机制研究
- 批准号:32000558
- 批准年份:2020
- 资助金额:24.0 万元
- 项目类别:青年科学基金项目
高效率单细胞分析微流控芯片的机理研究
- 批准号:31970754
- 批准年份:2019
- 资助金额:58.0 万元
- 项目类别:面上项目
核纤层蛋白维系染色体结构与调控基因表达的分子机理
- 批准号:31970752
- 批准年份:2019
- 资助金额:58.0 万元
- 项目类别:面上项目
基于新生血管显像研究MSC治疗缺血性脑血管病的转化医学关键问题
- 批准号:81171370
- 批准年份:2011
- 资助金额:58.0 万元
- 项目类别:面上项目
骨骼肌特定磷代谢物分子的影像学方法研究
- 批准号:81171339
- 批准年份:2011
- 资助金额:14.0 万元
- 项目类别:面上项目
相似海外基金
Three-dimensional fluorescence imaging flow cytometry at up to million frames per second
每秒高达百万帧的三维荧光成像流式细胞术
- 批准号:
10568627 - 财政年份:2023
- 资助金额:
-- - 项目类别:
Optimization of aminolevulinic acid-protoporphyrin IX for fluorescence-guided tumor resection and treatment
荧光引导肿瘤切除和治疗中氨基乙酰丙酸-原卟啉 IX 的优化
- 批准号:
10360122 - 财政年份:2022
- 资助金额:
-- - 项目类别:
Photonics-based Fluorescence Imaging for Research, Diagnostics, and Pathology
用于研究、诊断和病理学的基于光子学的荧光成像
- 批准号:
10546493 - 财政年份:2022
- 资助金额:
-- - 项目类别:
Photonics-based Fluorescence Imaging for Research, Diagnostics, and Pathology
用于研究、诊断和病理学的基于光子学的荧光成像
- 批准号:
10329143 - 财政年份:2022
- 资助金额:
-- - 项目类别:
Optimization of aminolevulinic acid-protoporphyrin IX for fluorescence-guided tumor resection and treatment
荧光引导肿瘤切除和治疗中氨基乙酰丙酸-原卟啉 IX 的优化
- 批准号:
10705406 - 财政年份:2022
- 资助金额:
-- - 项目类别:
A Novel Fluorescence Imaging Platform to Predict Response to Combinatorial Tyrosine Kinase Inhibitors
预测组合酪氨酸激酶抑制剂反应的新型荧光成像平台
- 批准号:
10356738 - 财政年份:2022
- 资助金额:
-- - 项目类别:
Fluorescence-based detection of inflammation and necrosis to inform surgical decision-making and enhance outcomes
基于荧光的炎症和坏死检测,为手术决策提供信息并提高结果
- 批准号:
10797980 - 财政年份:2022
- 资助金额:
-- - 项目类别:
Development of a 3D fluorescence lifetime imaging flow cytometer and analysis of drug response in cancer cell populations
开发 3D 荧光寿命成像流式细胞仪并分析癌细胞群中的药物反应
- 批准号:
21J10600 - 财政年份:2021
- 资助金额:
-- - 项目类别:
Grant-in-Aid for JSPS Fellows