A High Throughput System for Multichannel Neurophysiology

描述(申请人提供)：最近的技术发展使同时记录许多神经元的活动成为可能。这些实验在基础和应用研究方面都显示出巨大的前景，展示了大量神经元之间的动态相互作用，以及对假肢的神经假体控制或恢复感觉输入的潜力。为了支持这项研究，我们将基于我们现有的System 3硬件和软件开发一个非常高吞吐量的多通道神经生理学系统。这一新设计将利用我们现有系统设计的最佳优势，消除目前的瓶颈。该项目的最终目标是：(1)通过生产专用系统来简化数据采集，该系统旨在处理和记录由单台监视器PC控制和分析的多个通道的神经生理数据，以及(2)通过在新型专用数字信号处理(DSP)平台上实现高效的实时信号调理、尖峰分类、数据显示和分析，最大限度地减少对这些超大数据集的事后分析。本文提出的高通量数据采集平台旨在支持和扩展高计数多通道调查。我们的DSP架构非常适合于这种处理，作为我们在此提出的系统的基础，它将成为从复杂或高通道数神经生理学记录系统获取和预处理大量派生数据集的使能技术。高通量神经生理学系统的成功交付将提高数据产量、分析速度、减少动物使用，并允许解决有关同时神经活动和功能连接的基本问题。

DESCRIPTION (provided by applicant): Recent technical developments have made simultaneously recording the activity of many neurons possible. These experiments show great promise in both basic and applied research, illustrating the dynamic interplay between large populations of neurons as well as the potential for neuroprosthetic control of artificial limbs or restoring sensory input. To support this research, we will develop a very high throughput system for multichannel neurophysiology based on our existing System 3 hardware and software. This new design will utilize the best advantages of our existing system design and eliminate current bottlenecks. Our ultimate goals for this project are: (1) to streamline data acquisition by producing a dedicated system designed to process and record many channels of neurophysiological data, controlled and analyzed by a single monitor PC, and (2) to minimize post-hoc analysis of these very large data sets by allowing for efficient real-time signal conditioning, spike sorting, data display, and analysis on a novel new dedicated digital signal processing (DSP) platform. The high throughput data acquisition platform proposed here is designed to support and extend high-count multichannel investigations. Our DSP architecture is very well suited for this processing and as a foundation to the system we propose here, will serve as an enabling technology for acquiring and pre-processing large numbers of derived data sets from complex or high channel count neurophysiology recording systems. The successful delivery of a high throughput system for neurophysiology will increase data yield, speed analysis, reduce animal use, and allow fundamental questions about simultaneous neural activity and functional connectivity to be addressed.