Beta cell antigen presentation in models of Beta cell a*

Beta 细胞 a* 模型中的 Beta 细胞抗原呈递

• 批准号: 7038622
• 负责人: MICHIKO SHIMODA
• 金额: $ 7.31万
• 依托单位: AUGUSTA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7038622
• 关键词: antigen presentation; memory; model

DESCRIPTION (provided by applicant): Systemic lupus erythematosus (SLE) is a chronic, multisystem human autoimmue disease characterized by the differentiation of short- and long-lived plasma cells (PCs) that secrete autoantibodies. Although the exact cause of SLE is unclear, environmental factors such as polyclonal B cell activation by bacterial and/or viral infection seem to play a significant role in the emergence of disease. In this case, it is anticipated that activated autoreactive B cells may participate in germinal center reaction and remain as memory cells long after infection, which may give rise to long-lived PCs secreting autoantibodies. We propose a hypothesis that memory B cells can differentiate into PCs only when receiving CD40/CD40L signals by antigen presentation to T cells. However, immunomodulatory factors such as CpG DNA may bypass this pathway, which potentially results in generation of autoreactive long-lived PC. We recently generated IA-B mice that lack MHC-II on about 95% of all B cells due to B-cell-restricted deletion of a loxP-flanked iab-neo allele by the cd19cre (Cre recombinase) transgene. Upon immunization with a T cell dependent antigen, a small number of antigen-specific MHC-II+ B cells in IA-B mice dramatically expand to differentiate into GC B cells and make normal levels of B220+ CD38+ memory B cells. However, these memory B cells lose MHC-II expression later because of ongoing deletion of MHC-II by the cd19cre transgene. In association with loss of MHC-II on memory B cells, IA-B mice showed impaired affinity maturation in long-lived PCs. With use of IAB mice, the specific aims to test our hypothesis are: 1) determination of the role of CD40/CD40L signal on memory B cell differentiation to long-lived PC by using IA-B mice carrying B cell specific CD40L transgene, and 2) determination of the effect of immunomodulatory factors that can bypass the requirement of MHC-II dependent antigen-presentation to T cells in long-lived PC differentiation. The outcome will provide a great help for understanding the development of autoreactive long-lived PCs and create new avenues for exploring therapy for SLE patients.

描述(申请人提供)：系统性红斑狼疮(SLE)是一种慢性、多系统的人类自身免疫性疾病，其特征是分泌自身抗体的短期和长期浆细胞(PC)的分化。虽然SLE的确切原因尚不清楚，但环境因素，如细菌和/或病毒感染激活的多克隆B细胞，似乎在疾病的出现中发挥了重要作用。在这种情况下，预计激活的自身反应性B细胞可能参与生发中心反应，并在感染后很长时间保持记忆细胞，这可能导致长寿的PC分泌自身抗体。我们提出了一个假设，即记忆B细胞只有在接受CD40/CD40L信号时才能通过向T细胞递呈抗原而分化为PC。然而，免疫调节因子，如CpG DNA，可能绕过这一途径，这可能导致自身反应性长寿命PC的产生。我们最近产生了IA-B小鼠，由于B细胞通过cd19cre(Cre重组酶)转基因限制了loxP侧翼的IAB-neo等位基因的缺失，大约95%的B细胞上缺乏MHC-II。经T细胞依赖抗原免疫后，IA-B小鼠体内的少量抗原特异性MHC-II+B细胞急剧扩增，分化为GC B细胞，并使B220+CD38+记忆性B细胞水平正常。然而，这些记忆B细胞后来失去了MHC-II的表达，因为cd19cre转基因正在持续地删除MHC-II。与记忆B细胞上MHC-II的丢失有关，IA-B小鼠在长期存活的PC中显示出亲和力成熟受损。用IAB小鼠验证我们的假说的具体目的是：1)通过携带B细胞特异性CD40L转基因的IA-B小鼠，确定CD40/CD40L信号在记忆B细胞向长寿PC分化中的作用；2)确定在长寿PC分化过程中，免疫调节因子可以绕过MHC-II依赖的抗原提呈对T细胞的影响。这一结果将为了解自身反应性长寿命PC的发展提供很大帮助，并为探索SLE患者的治疗开辟新的途径。