Emory Epithelial Pathobiology Research Development Cent*

拟议的“埃默里上皮病理生物学研究发展中心”将包括3个核心，以支持由9名已建立的研究人员组成的13项NIH R01资助的研究项目，其中7项由NIDDK资助，横跨埃默里大学医学院的4个部门。此外，中心将支持5名被认为是新研究人员的初级成员的研究活动。这组研究人员共同的核心研究主题是了解消化道上皮的生物学和病理生物学。特别是，该中心支持的项目将集中于了解肠道上皮的生理、炎症和增殖反应。DDRDC申请的目标是为这群高度专注的研究人员提供共享的资源和设施，以提高研究能力，促进新的研究方向，并促进研究人员之间的强烈互动和合作。中心拟建的核心有：(A)基因表达分析核心，(B)图像分析核心，(C)细胞培养和单克隆抗体核心。核心A将包括一个具有生物信息学和生物统计学支持的专用cDNA微阵列设备，并将专注于与消化系统上皮生物学和病理生物学相关的基因表达谱。核心B将包括最先进的共聚焦显微镜和荧光激活细胞分选器，以提高研究人员的图像分析能力。Core C将专注于为研究人员提供专门的可渗透培养支持（transwells），用于涉及极化上皮表型的实验，以及支持单克隆抗体产生的设施。这三个核心都将位于埃默里大学主校区新建成的Whitehead生物医学研究大楼。大多数参与调查者的实验室位于同一栋楼内，其余实验室位于容易到达的距离内。预计在拟议的核心设施的支持下，该中心将大大提高参与研究人员的研究能力，并有助于进一步促进埃默里大学强大的上皮生物学和病理生物学小组的发展，并在国家糖尿病、消化和肾脏疾病研究所（NIDDK）的支持下，在任务范围内开展强有力的研究。

The proposed "Emory Epithelial Pathobiology Research Development Center" will include 3 cores to support the research programs by a collect group of 9 established investigators with a composite of 13 NIH R01 grants, 7 of which are funded by the NIDDK, and spans across 4 departments of the Emory University School of Medicine. In addition, the Center will support the research activities of 5 junior members who are considered new investigators. This group of investigators shares a central research theme of understanding the biology and pathobiology of the epithelium in the digestive tract. In particular, projects to be supported by the Center will concentrate on understanding the physiologic, inflammatory and proliferative responses of the gut epithelium. The GOAL of the proposed DDRDC application is to provide shared resources and facilities for this group of highly focused investigators in order to enhance research capability, promote new research directions, and foster strong interactions and collaborations among the researchers. The proposed cores of the Center are: (A) Gene Expression Analysis Core, (B) Image Analysis Core, and (C) Cell Culture and Monoclonal Antibody Core. Core A will include a dedicated cDNA microarray facility with bioinformatic and biostatistical support and will focus on the profiling of gene expression relevant to epithelial biology and pathobiology of the digestive system. Core B will include a state-of-the-art confocal microscope and a fluorescence activated cell sorter to enhance investigators' ability for image analysis. Core C will focus on providing investigators specialized permeable culture support (transwells) for experiments involving the polarized epithelial phenotype as well as a facility to support the generation of monoclonal antibodies. All three cores will be located in the newly completed Whitehead Biomedical Research Building on the main campus of the Emory University. The majority of participating investigators' laboratories are located in the same building and the remainders are located within easily accessible distance. It is anticipated that with the support of the proposed core facilities, the Center will greatly enhance the research capabilities of the involved investigators and help further foster the development of a strong epithelial biology and pathobiology group at Emory with a strong research focus on areas within the mission supported by the National Institute of Diabetes, Digestive and Kidney Diseases (NIDDK).