Evolution of Novel Farnesyltransferase Activity
新型法尼基转移酶活性的演变
基本信息
- 批准号:7158273
- 负责人:
- 金额:$ 4.6万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2006
- 资助国家:美国
- 起止时间:2006-08-01 至 2009-01-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
DESCRIPTION (provided by applicant): Prenylation is an important posttranslational modification for many proteins whereby attachment of a lipid tail leads to protein localization to cellular membranes. The two enzymes responsible for the majority of prenylation, farnesyltransferase (FTase) and geranylgeranyltransferase type I (GGTase-l), have been proposed to recognize a consensus "CaaX" box motif at the C-terminus of target proteins. However, recent work has shown that this model incompletely describes the specificity of these enzymes. Furthermore, the interactions between the protein substrates and FTase responsible for specificity have not been delineated. I will investigate these issues using mutagenesis and directed evolution to generate a library of FTase variants with altered substrate specificities and kinetic behavior. Analysis of these mutated FTases will provide a more comprehensive understanding of the features that govern binding and catalysis within FTase and may aid in the identification of novel substrates and design of new FTase inhibitors as therapeutic agents. In addition, this work will provide important information and novel tools for studying protein prenylation in vivo, allowing examination of the myriad biological roles of prenylated proteins.
描述(申请人提供):预基化是许多蛋白质的一种重要的翻译后修饰,通过附着脂尾导致蛋白质定位到细胞膜上。负责苯丙基化的两种酶,法尼基转移酶(FTase)和香叶基香叶基转移酶I型(GGTase-L),被认为是识别靶蛋白C-末端一致的“CAAX”框基序。然而,最近的工作表明,这个模型不能完全描述这些酶的特异性。此外,负责特异性的蛋白质底物和FTase之间的相互作用还没有被描述。我将使用突变和定向进化来研究这些问题,以生成具有改变底物特异性和动力学行为的FTase变异体库。对这些突变的FTase的分析将提供一个更全面的了解控制FTase结合和催化的特征,并可能有助于识别新的底物和设计新的FTase抑制剂作为治疗剂。此外,这项工作将为研究体内蛋白质的预烯基化提供重要的信息和新的工具,使我们能够研究预烯基化蛋白质的各种生物学作用。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
James Hougland其他文献
James Hougland的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('James Hougland', 18)}}的其他基金
FASEB SRC: The Protein Lipidation Conference: Enzymology, Signaling, and Therapeutics
FASEB SRC:蛋白质脂化会议:酶学、信号传导和治疗学
- 批准号:
10468574 - 财政年份:2022
- 资助金额:
$ 4.6万 - 项目类别:
相似海外基金
Bridging the Gap: Next-Gen Tools for Accurate Prediction of Disordered Protein Binding Sites
弥合差距:准确预测无序蛋白质结合位点的下一代工具
- 批准号:
24K15172 - 财政年份:2024
- 资助金额:
$ 4.6万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Design of protein crystal templates with multiple binding sites for tracking metal complex reactions.
设计具有多个结合位点的蛋白质晶体模板,用于跟踪金属络合物反应。
- 批准号:
23K04928 - 财政年份:2023
- 资助金额:
$ 4.6万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Dynamic changes in PIP2 binding sites and their impact on axonal targeting and function of epilepsy-associated KCNQ/Kv7 channels
PIP2 结合位点的动态变化及其对癫痫相关 KCNQ/Kv7 通道的轴突靶向和功能的影响
- 批准号:
10744934 - 财政年份:2023
- 资助金额:
$ 4.6万 - 项目类别:
Computational methods to identify small molecule RNA binding sites
识别小分子 RNA 结合位点的计算方法
- 批准号:
573688-2022 - 财政年份:2022
- 资助金额:
$ 4.6万 - 项目类别:
University Undergraduate Student Research Awards
Identification of potential drug binding sites within allosteric networks in cyclic nucleotide modulated channels
环核苷酸调节通道变构网络内潜在药物结合位点的鉴定
- 批准号:
10704557 - 财政年份:2022
- 资助金额:
$ 4.6万 - 项目类别:
Identification of potential drug binding sites within allosteric networks in cyclic nucleotide modulated channels
环核苷酸调节通道变构网络内潜在药物结合位点的鉴定
- 批准号:
10537846 - 财政年份:2022
- 资助金额:
$ 4.6万 - 项目类别:
Identifying new types of inhibitors in quinone binding sites in photosynthetic enzymes
鉴定光合酶醌结合位点的新型抑制剂
- 批准号:
2753921 - 财政年份:2022
- 资助金额:
$ 4.6万 - 项目类别:
Studentship
Development of broad nanovaccines targeting diverse coronavirus receptor-binding sites
开发针对不同冠状病毒受体结合位点的广泛纳米疫苗
- 批准号:
10328140 - 财政年份:2022
- 资助金额:
$ 4.6万 - 项目类别:
Exploiting Water Network Perturbations in Protein Binding Sites
利用蛋白质结合位点的水网络扰动
- 批准号:
10621368 - 财政年份:2021
- 资助金额:
$ 4.6万 - 项目类别:
SBIR Phase I: Nonlinear optical method for identifying protein-ligand binding sites
SBIR 第一阶段:识别蛋白质-配体结合位点的非线性光学方法
- 批准号:
2111821 - 财政年份:2021
- 资助金额:
$ 4.6万 - 项目类别:
Standard Grant