Interaction of auxin and abscisic acid signaling

描述(由申请人提供)：这个项目的长期目标是加强我们对植物激素生长素和脱落酸之间的信号串扰的理解。最近克隆的IBR5编码一个假定的双特异性磷酸酶，也是这项建议的主题，揭示了生长素和ABA信号转导之间的一个新的相互作用点。几种方法将使用IBR5来更好地了解生长素和ABA之间的关系。首先，将分离ibr5生长素不敏感和ABA不敏感的抑制子(目标1)。将对这些抑制子进行表型鉴定(Aim 2)，并克隆有缺陷的基因(Aim 3)。至少这些抑制子中的一些有望携带IBR5底物基因的损伤。抑制子的克隆和免疫共沉淀将被用于确定潜在的IBR5底物(目标4)。通过研究和克隆抑制子，以及鉴定IBR5底物，将对生长素和ABA串扰有更深入的了解。

DESCRIPTION (provided by applicant): The long-term goal of this project is to enhance our understanding of the signaling cross-talk between the phytohormones auxin and ABA. The recent cloning of IBR5, encoding a putative dual-specificity phosphatase and the subject of this proposal, revealed a novel point of interaction between auxin and ABA signal transduction. Several approaches will use IBR5 to gain greater understanding of the relationship between auxin and ABA. Firstly, suppressors if ibr5 auxin insensitivity and ABA insensitivity will be isolated (Aim 1). These suppressors will be phenotypically characterized (Aim 2) and the defective genes will be cloned (Aim 3). At least some of these suppressors are expected to carry lesions in IBR5 substrate genes. Both cloning of suppressors and co-immunoprecipitation will be used to identify potential IBR5 substrates (Aim 4). By studying and cloning suppresors, as well as identifying IBR5 substrates, a more in-depth understanding of auxin and ABA cross-talk will be gained.