Interaction of auxin and abscisic acid signaling

项目描述（申请人提供）：该项目的长期目标是加强我们对植物激素生长素和ABA之间信号串扰的理解。最近克隆的IBR5编码一种假定的双特异性磷酸酶，揭示了生长素和ABA信号转导之间的一个新的相互作用点。有几种方法将使用IBR5来更好地了解生长素和ABA之间的关系。首先，分离ibr5生长素不敏感和ABA不敏感的抑制因子（Aim 1）。这些抑制基因将被表型表征（目的2），缺陷基因将被克隆（目的3）。这些抑制基因中至少有一些可能携带IBR5底物基因的病变。克隆抑制子和共免疫沉淀将用于鉴定潜在的IBR5底物（目的4）。通过对抑制因子的研究和克隆，以及对IBR5底物的鉴定，将对生长素和ABA的串扰有更深入的了解。

DESCRIPTION (provided by applicant): The long-term goal of this project is to enhance our understanding of the signaling cross-talk between the phytohormones auxin and ABA. The recent cloning of IBR5, encoding a putative dual-specificity phosphatase and the subject of this proposal, revealed a novel point of interaction between auxin and ABA signal transduction. Several approaches will use IBR5 to gain greater understanding of the relationship between auxin and ABA. Firstly, suppressors if ibr5 auxin insensitivity and ABA insensitivity will be isolated (Aim 1). These suppressors will be phenotypically characterized (Aim 2) and the defective genes will be cloned (Aim 3). At least some of these suppressors are expected to carry lesions in IBR5 substrate genes. Both cloning of suppressors and co-immunoprecipitation will be used to identify potential IBR5 substrates (Aim 4). By studying and cloning suppresors, as well as identifying IBR5 substrates, a more in-depth understanding of auxin and ABA cross-talk will be gained.