Chondrogenesis in Enzymatically Degradable Hydrogels

酶降解水凝胶中的软骨形成

• 批准号: 7023784
• 负责人: Jason A Burdick
• 金额: $ 13.5万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-03-05 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7023784
• 关键词: athymic mouse; biodegradable product; bioengineering /biomedical engineering; biomaterial development /preparation; biomaterials; bone marrow; cartilage development; cartilage disorder; chondrocytes; copolymer; gel; nonhuman therapy evaluation; photochemistry; polymerization; polymethacrylate; protein degradation; stem cells; swine; tissue /cell culture; tissue engineering; transforming growth factors

DESCRIPTION (provided by applicant): The overall purpose of this K22 Scholar Development and Faculty Transition Career Award is to provide additional (1 year) postdoctoral mentorship and support to the principle investigator in the area of advancing photopolymerizable hydrogels for cartilage tissue engineering in addition to support (4 years) as an independent faculty member to continue research in this field. The proposed research originated to complement the principle investigator's background in the development of novel photocrosslinkable biomaterials for bone tissue engineering with a long-term objective of developing complex materials at the interface of bone and cartilage for craniofacial applications. It is the general goal of the principle investigator to develop an internationally recognized research laboratory that uses the synthesis and modification of novel materials as a platform for the development of clinically relevant therapeutics. The proposed research and career development program outlined in this proposal were developed by the principle investigator and Mentors from both the Massachusetts General Hospital and Massachusetts Institute of Technology in an effort to achieve this goal. Photopolymerizable hydrogels have many properties that are beneficial for cartilage tissue engineering including: the ability to form in vivo to fill complex defects with potential adhesive and integrative properties, both spatial and temporal control over the polymerization process, and biological signals are readily incorporated into the networks to control both cellular differentiation and tissue formation. These properties would be extremely beneficial for the regeneration of cartilage in craniofacial reconstruction. With these benefits in mind, the primary aims of the proposed research are to: . synthesize and characterize photopolymerizable copolymer hydrogel networks that resorb via an enzymatic degradation process, . investigate the quantity, distribution, and type of tissue formed by chondrocytes encapsulated in hydrogel networks as a function of hydrogel composition, . incorporate signals into hydrogel networks to control the chondrogenic differentiation of photoencapsulated mesenchymal stem cells, and . analyze critical issues of the photopolymerized constructs necessary for clinical application of these techniques.

描述（由申请人提供）：该K22学者发展和教师过渡职业奖的总体目的是为额外的（1年）提供多（1年）的博士后指导和支持，以推进针对软骨组织工程的可推进光聚糖水凝胶的领域，除了支持（4年）作为独立的教职员工成员，以继续研究该领域。拟议的研究起源是为了补充主要研究者在开发骨组织工程的新型光叠型生物材料中的背景，其长期目标是在颅骨的骨骼和软骨界面上开发复杂的材料，以用于颅面应用。原则研究者的一般目标是开发国际认可的研究实验室，该研究实验室使用新型材料的合成和修饰作为开发临床相关治疗剂的平台。该提案中概述的拟议的研究和职业发展计划是由马萨诸塞州综合医院和马萨诸塞州理工学院的主要研究者和指导者制定的，以实现这一目标。 可光聚合的水凝胶具有许多对软骨组织工程有益的特性，包括：在体内形成具有潜在的粘合剂和积分特性的复杂缺陷的能力，对聚合过程进行了空间和时间控制，并且生物学信号都易于整合到网络中，以控制细胞分化和组织组成和组织组成。这些特性将对颅面重建中软骨的再生非常有益。考虑到这些好处，拟议研究的主要目的是： 。 合成和表征可通过酶促降解过程吸收的可光聚合共聚物水凝胶网络， 。 研究由水凝胶网络中的软骨细胞形成的组织的数量，分布和类型，这是水凝胶组成的函数， 。 将信号纳入水凝胶网络中，以控制光囊化间充质干细胞的软骨分化和 。 分析这些技术临床应用所需的光聚合构建体的关键问题。