Core--ANIMAL AND CELL MODEL

核心--动物和细胞模型

• 批准号: 7005298
• 负责人: James J Manfredi
• 金额: $ 8.75万
• 依托单位: ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7005298
• 关键词: animal breeding; animal colony; biomedical facility; cell line; cellular oncology; genetic models; genetic regulation; genetically modified animals; histopathology; laboratory mouse; neoplasm /cancer genetics; p53 gene /protein; serial analysis of gene expression; tissue /cell preparation

Insights gained primarily from in vitro models of p53 regulators and effectors within our research program have led to a critical need for in vivo validation and the ability to gain further understanding using in vivo models. The phenotypes that are actively being examined in the p53 paradigm are no longer simply a matter of cell death, growth arrest or senescence. Instead, the role of p53 activation in vivo will likely have different consequences in different cell and tissue types and will likely differ with regard to developmental stages. This Core maintains stocks of human and animal tumor cell lines as well as genetically engineered cell lines that are used within the Program. The Core also derives and maintains adequate stocks of early passage aliquots of frozen primary cells derived from mouse strains for distribution to the investigators in the Program. The research efforts of our productive and collaborative program greatly benefit from an in vivo models component, which maintains small breeding colonies of critically needed mouse strains for genetic and cell biologic investigations by our investigators. The high cost of animal studies in barrier facilities can be reduced substantially by the availability of shared technical support for mouse breeding and genetic analyses and for shared immuno-histochemistry. The cost effectiveness is further emphasized by the shared use of the same genetically modified strains by several investigators and projects within the Program. The Cell and Animal Model Core will derive and maintain human and mouse cell lines as well as adequate stocks of early passage aliquots of frozen primary cells utilized by the projects. The Core will also acquire and maintain steadily used mouse models (transgenic, knockouts, etc.) and primary cells derived from these mice for distribution to the investigators in the Program. Lastly, the core will provide expertise and guidance in the preparation of tissue and/or embryos for analyses of gene expression (protein and mRNA in situ) and histopathology.

我们的研究计划中主要从p53调节器和效应子的体外模型中获得的见解导致了对体内验证的迫切需求，并且使用体内模型获得了进一步理解的能力。在p53范式中积极检查的表型不再仅仅是细胞死亡，生长停滞或衰老的问题。取而代之的是，p53激活在体内的作用可能在不同的细胞和组织类型中会产生不同的后果，并且在发育阶段可能会有所不同。该核心维持人类和动物肿瘤细胞系的库存以及该程序中使用的基因工程细胞系。该核心还得出并维持足够的早期通过等分试样的库存，这些库存是从小鼠菌株中得出的冷冻原代细胞的，用于分布到该程序中的研究者。我们的生产力和协作计划的研究工作从体内模型组件中受益匪浅，该模型的组成部分维持了急需的小鼠菌株的小型繁殖菌落，用于我们的研究人员进行遗传和细胞生物学研究。通过对小鼠育种和遗传分析的共同技术支持以及共享的免疫 - 归化学，可以大大降低障碍设施中动物研究的高成本。共同使用的成本效益进一步强调了 该计划中的几个调查人员和项目对基因修饰的菌株进行了相同的遗传菌株。 细胞和动物模型核心将得出并维持人和小鼠细胞系以及 项目利用的冷冻原代细胞的早期通过等分试样的足够库存。核心还将获取并维持稳定使用的小鼠模型（转基因，敲除等），以及从这些小鼠中得出的原代细胞，以分配给程序中的研究者。最后，核心将在制备组织和/或胚胎的基因表达（原位蛋白质和mRNA）和组织病理学的分析方面提供专业知识和指导。