Structure And Stabilization Of The Bacterial Nucleoid

细菌核的结构和稳定性

• 批准号: 7152618
• 负责人: Steven C. Zimmerman
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7152618
• 关键词: Escherichia coli; bacterial DNA; bacterial RNA; bacterial genetics; bacterial proteins; cell component structure /function; cell nucleus; chloramphenicol; fluorescence microscopy; formaldehyde; molecular /cellular imaging; nucleic acid denaturation; nucleoproteins; phase contrast microscopy; polylysine; protein sequence; spermidine; tissue /cell preparation

The genomic DNA of Escherichia coli occurs in compact bodies known as nucleoids. Organization and structure of nucleoids are poorly understood. Compact, characteristically-shaped, nucleoids isolated by the polylysine-spermidine procedure were visualized by DNA fluorescence microscopy. Treatment with urea or trypsin converted compact nucleoids to partially expanded forms. The abrupt transition that occurred in urea solutions was accompanied by release of most DNA-associated proteins; the transition point between compact and partially expanded forms was not changed by the loss of the proteins nor was it changed in nucleoids isolated from cells after exposure to chloramphenicol or from cells in which Dps, Fis, or H-NS and StpA had been deleted. Partially expanded forms that had been stripped of most DNA-associated proteins were efficiently recompacted by polyethylene glycol 8000, a macromolecular crowding agent, in an abrupt transition. DNA-associated proteins are accordingly suggested to have relatively little effect on the phase-like behavior of the cellular nucleoid. Partially expanded forms became dispersed upon RNase exposure, indicating a role of RNA in maintaining the partial expansion. Changes in the urea transition indicate that a previously described procedure for nucleoid compaction may have an artifactual basis, and raise questions about reports of repetitive local structures involving the DNA of lysed cells. This material has been prepared and submitted for publication.

大肠杆菌的基因组DNA存在于被称为类核的致密体中。类核的组织和结构知之甚少。通过DNA荧光显微镜观察由聚赖氨酸-亚精胺程序分离的紧凑的、特征性形状的类核。用尿素或胰蛋白酶处理将紧凑的类核转化为部分扩展的形式。尿素溶液中发生的突然转变伴随着大多数DNA相关蛋白质的释放;蛋白质的丢失不会改变紧凑和部分扩展形式之间的转变点，也不会改变从暴露于氯霉素后的细胞或从Dps，Fis或H-NS和StpA已被删除的细胞中分离的类核。部分扩展的形式，已被剥夺了大多数DNA相关的蛋白质，有效地重新压缩聚乙二醇8000，大分子拥挤剂，在一个突然的过渡。因此，DNA相关蛋白对细胞类核的类相行为的影响相对较小。部分扩展的形式变得分散在RNase曝光，表明RNA在维持部分扩展的作用。尿素转换的变化表明，先前描述的程序的类核压实可能有一个人为的基础，并提出了问题的重复性的局部结构的报告，涉及裂解细胞的DNA。这份材料已经编写并提交出版。

项目成果

Is opium a real risk factor for esophageal cancer or just a methodological artifact? Hospital and neighborhood controls in case-control studies.

• DOI: 10.1371/journal.pone.0032711
• 发表时间: 2012
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Shakeri R;Kamangar F;Nasrollahzadeh D;Nouraie M;Khademi H;Etemadi A;Islami F;Marjani H;Fahimi S;Sepehr A;Rahmati A;Abnet CC;Dawsey SM;Brennan P;Boffetta P;Malekzadeh R;Majdzadeh R
• 通讯作者: Majdzadeh R

Are Women Who Smoke at Higher Risk for Lung Cancer Than Men Who Smoke?

• DOI: 10.1093/aje/kws445
• 发表时间: 2013-04-01
• 期刊: AMERICAN JOURNAL OF EPIDEMIOLOGY
• 影响因子: 5
• 作者: De Matteis, Sara;Consonni, Dario;Landi, Maria Teresa
• 通讯作者: Landi, Maria Teresa

Re: more data regarding the effects of passive smoking on breast cancer risk among younger women.

回复：有关被动吸烟对年轻女性乳腺癌风险影响的更多数据。

• DOI: 10.1002/ijc.22610
• 发表时间: 2007
• 期刊: International journal of cancer
• 影响因子: 6.4
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Release of compact nucleoids with characteristic shapes from Escherichia coli.

从大肠杆菌中释放具有特征形状的紧凑核仁。

• DOI: 10.1128/jb.183.17.5041-5049.2001
• 发表时间: 2001
• 期刊: Journal of bacteriology
• 影响因子: 3.2
• 作者: Zimmerman,SB;Murphy,LD
• 通讯作者: Murphy,LD

Underlying regularity in the shapes of nucleoids of Escherichia coli: implications for nucleoid organization and partition.

大肠杆菌核形状的基本规律：对核组织和分区的影响。

• DOI: 10.1016/s1047-8477(02)00637-8
• 发表时间: 2003
• 期刊: Journal of structural biology
• 影响因子: 3
• 作者: Zimmerman,StevenB