Liquid-liquid phase-separation of small molecules and proteins

小分子和蛋白质的液-液相分离

• 批准号: 2821139
• 金额: --
• 依托单位: University of Edinburgh
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2821139
• 关键词: Liquid; liquid; phase; separation; small

From forming membrane-less compartments inside cells to precursors during crystallisation processes, liquid-liquid phase separation of ions, small molecules and proteins is a fundamental process in both biological and synthetic systems. The project in this studentship will build on recent preliminary findings from both the Nudelman and Alexander groups where small organic molecules were found to form dense liquid phases in solution before crystallizing. We will study the driving force and the mechanisms of this process, going from small molecules to proteins known to form liquid droplets inside cells.This project will provide training in a range of materials characterization techniques including light and electron microscopy, X-ray and electron diffraction, dynamic light scattering and infra-red spectroscopy.

从在细胞内形成无膜隔室到结晶过程中的前体，离子、小分子和蛋白质的液-液相分离是生物和合成系统中的基本过程。这个学生项目将建立在Nudelman和亚历山大小组最近的初步发现基础上，这些小组发现小有机分子在结晶之前在溶液中形成致密的液相。我们将研究这一过程的驱动力和机制，从小分子到已知在细胞内形成液滴的蛋白质。该项目将提供一系列材料表征技术的培训，包括光学和电子显微镜，X射线和电子衍射，动态光散射和红外光谱。