Novel Method for Sample Processing

样品处理新方法

• 批准号: 7081153
• 负责人: Yajuan Jiang
• 金额: $ 14.99万
• 依托单位: ONE CELL SYSTEMS, INC
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-01 至 2007-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7081153
• 关键词: flow cytometry; immunophenotype; intracellular

DESCRIPTION (provided by applicant): Development of improved procedures for sample preparation and storage of biological specimens are critical for diagnosis of human diseases, particularly cancer, for which positive cells are rare. Recent improvements in sample preparation and storage of cervical specimens led to significant increase in sensitivity and accuracy of cervical cancer screening. Although important, there have been few improvements in sample preparation, handling and storage of other biological specimens (e.g. fine needle aspirates or other biopsy materials). Problems associated with specimen handling include cell loss and cell clumping. Sample loss resulting from conventional biological specimen processing conditions such as fixation and permeabilization, used for intracellular immunophenotyping, is a well documented phenomenon. If the sample is limited, or cells of interest are rare, cell loss is a significant problem hampering research, diagnosis, patient monitoring, and clinical studies. This SBIR aims to develop a simple procedure to reduce cell loss associated with sample processing and intracellular immunophenotyping, as well as cell clumping.

描述（由申请人提供）：开发改进的样品制备和生物标本储存程序对于诊断人类疾病，特别是癌症至关重要，因为阳性细胞很少。最近在宫颈标本制备和储存方面的改进导致宫颈癌筛查的敏感性和准确性显著提高。虽然很重要，但在样品制备、处理和其他生物标本（例如细针抽吸器或其他活检材料）的储存方面几乎没有改进。与标本处理相关的问题包括细胞丢失和细胞结块。用于细胞内免疫表型分析的常规生物标本处理条件（如固定和渗透）导致的样品损失是一个有充分记录的现象。如果样本有限，或者感兴趣的细胞很少，细胞丢失是阻碍研究、诊断、患者监测和临床研究的一个重要问题。该SBIR旨在开发一种简单的程序，以减少与样品处理和细胞内免疫分型以及细胞聚集相关的细胞损失。