Novel Stem Cell Assay

新型干细胞检测

• 批准号: 7220515
• 负责人: Yajuan Jiang
• 金额: $ 14.8万
• 依托单位: ONE CELL SYSTEMS, INC
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2008-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7220515
• 关键词: Adoption; Animal Model; Biological Assay; CD34 gene; Cell Count; Cell Lineage; Cells; Chronic; Colony-Forming Units Assay; Colony-forming units; Cultured Cells; Data; Development; Disease; Encapsulated; End stage renal failure; Engraftment; Erythroid; Evaluation; Flow Cytometry; Frequencies; Gel; Government; Growth; Heart Diseases; Human; Incubated; Left; Liver Failure; Magnetism; Malignant Neoplasms; Marines; Megakaryocytes; Methods; Methylcellulose; Microscopic; Microscopy; Microspheres; Monitor; Mononuclear; Morphology; Myelogenous; Persons; Phase; Population; Proliferating; Property; Publishing; Purpose; Reagent; Research; Research Personnel; Score; Scoring Method; Sepharose; Side; Small Business Funding Mechanisms; Small Business Innovation Research Grant; Sorting - Cell Movement; Staining method; Stains; Stem cells; Surface; System; Technology; Therapeutic; Time; Umbilical Cord Blood; base; cell fixation; clinical application; concept; cytotoxicity; day; gel microdrop encapsulation; interest; light scattering; microorganism; novel; progenitor; rapid technique

DESCRIPTION (provided by applicant): Although research and clinical applications for stem cells have increased dramatically in the last decade, development of new assay formats and reagents has not kept pace. The current Colony Forming Cell (CFC) assay, which relies on colony evaluation and enumeration by microscopy after culture on plates, is slow with a cell culture period of about 14-16 days. The microscopic scoring method used in this assay format also limits the number of cells that can be screened. Using single cell gel microdrop (GMD) encapsulation technology and flow cytometry, this SBIR aims to develop a rapid, quantitative assay to simultaneously detect colony formation and identify cell lineage. Using this assay format, sub-populations of cells of interest can be isolated, and propagated for further studies and use in therapeutic applications. The proposed assay will provide a rapid method for identifying and isolating sub-populations of stem cells of interest for use in research and clinical applications, including cellular therapies for diseases such as chronic heart disease, end-stage kidney disease, liver failure and cancer.

描述（由申请人提供）：尽管干细胞的研究和临床应用在过去十年中急剧增加，但新的检测形式和试剂的开发没有跟上。目前的集落形成细胞（CFC）测定依赖于在平板上培养后通过显微镜进行的集落评价和计数，其缓慢，细胞培养期约为14-16天。该测定形式中使用的显微镜评分方法也限制了可筛选的细胞数量。使用单细胞凝胶微滴（GMD）包封技术和流式细胞术，该SBIR旨在开发一种快速，定量的检测方法，以同时检测集落形成和鉴定细胞谱系。使用这种测定形式，可以分离和繁殖感兴趣的细胞亚群，用于进一步研究和治疗应用。所提出的测定将提供一种用于鉴定和分离感兴趣的干细胞亚群的快速方法，用于研究和临床应用，包括慢性心脏病、终末期肾病、肝衰竭和癌症等疾病的细胞疗法。