Method for Analyzing and Isolating Stem Cells

分析和分离干细胞的方法

• 批准号: 7157071
• 负责人: Yajuan Jiang
• 金额: $ 41.39万
• 依托单位: ONE CELL SYSTEMS, INC
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-01 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7157071
• 关键词: CD34 molecule; biomarker; cell differentiation; cell line; cell sorting; cell surface receptors; colony stimulating factor; cytokine; cytotoxicity; flow cytometry; gene expression; hematopoietic stem cells; immunophenotype; secretory protein; stem cells; technology /technique development; tumor necrosis factor beta

DESCRIPTION (provided by applicant): In recent years, it has become clear that the majority of cell surface markers are shared by many types of stem and progenitor cells. Thus the ability to separate unique cell sub-populations using current methods is limited. Although it is known that stem cells secrete specific factors into the extracellular space, it is difficult to characterize secretory activity of individual viable cells within a heterogeneous population. Association of the secreted factor(s) with a specific cell type provides an additional marker that can be used to characterize the cell and permit separation of cells while maintaining viability. Combining immunophenotyping with secretory profile is powerful approach for both research and eventual therapeutic applications. Development of new, reliable methods for analyzing and recovering single, viable stem cells based on secretory profile combined with expression of cell surface antigens is of considerable interest. The GMD Stem Cell Assay addresses the need for a functional, multiparameter, single cell assay that does not compromise cell viability, physiological state, and functionality. Phase I research optimized the assay for use with several experimental hematopoietic models. Phase II research will expand use of the assay with other models, including endothelial progenitor cells. This assay format can be widely used for immunological studies, including stem cell research and clinical applications. By providing a reliable method for analyzing and recovering single, viable stem cells based on secretory profile combined with expression of cell surface antigens, this assay format will be useful for stem cell research and clinical applications.

描述（由申请人提供）：近年来，已经清楚的是，大多数细胞表面标记是许多类型的干细胞和祖细胞共有的。因此，使用当前方法分离独特细胞亚群的能力是有限的。尽管已知干细胞将特定因子分泌到细胞外空间，但很难表征异质群体中单个活细胞的分泌活性。分泌因子与特定细胞类型的关联提供了额外的标记，可用于表征细胞并允许细胞分离，同时保持活力。将免疫表型分析与分泌谱相结合是研究和最终治疗应用的强大方法。基于分泌谱与细胞表面抗原的表达相结合，开发新的、可靠的方法来分析和回收单个、活的干细胞是相当令人感兴趣的。 GMD 干细胞测定满足了对功能性、多参数、单细胞测定的需求，该测定不会损害细胞活力、生理状态和功能。第一阶段研究优化了该检测方法，以用于多种实验性造血模型。 II 期研究将扩大该检测方法在其他模型中的应用，包括内皮祖细胞。这种检测形式可广泛用于免疫学研究，包括干细胞研究和临床应用。通过提供一种基于分泌谱与细胞表面抗原表达相结合的分析和回收单个活干细胞的可靠方法，这种测定形式将可用于干细胞研究和临床应用。