Method for Analyzing and Isolating Stem Cells

分析和分离干细胞的方法

• 批准号: 7280790
• 负责人: Yajuan Jiang
• 金额: $ 16.87万
• 依托单位: ONE CELL SYSTEMS, INC
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-01 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7280790
• 关键词: Address; Biological Assay; Cell Separation; Cell Survival; Cell surface; Cells; Colony-Forming Units Assay; Development; Extracellular Space; Hematopoietic; Immunophenotyping; Individual; Methods; Modeling; Phase; Physiological; Population; Population Heterogeneity; Research; Stem Cell Research; Stem cells; Surface Antigens; Therapeutic; base; cell type; clinical application; interest; stem

DESCRIPTION (provided by applicant): In recent years, it has become clear that the majority of cell surface markers are shared by many types of stem and progenitor cells. Thus the ability to separate unique cell sub-populations using current methods is limited. Although it is known that stem cells secrete specific factors into the extracellular space, it is difficult to characterize secretory activity of individual viable cells within a heterogeneous population. Association of the secreted factor(s) with a specific cell type provides an additional marker that can be used to characterize the cell and permit separation of cells while maintaining viability. Combining immunophenotyping with secretory profile is powerful approach for both research and eventual therapeutic applications. Development of new, reliable methods for analyzing and recovering single, viable stem cells based on secretory profile combined with expression of cell surface antigens is of considerable interest. The GMD Stem Cell Assay addresses the need for a functional, multiparameter, single cell assay that does not compromise cell viability, physiological state, and functionality. Phase I research optimized the assay for use with several experimental hematopoietic models. Phase II research will expand use of the assay with other models, including endothelial progenitor cells. This assay format can be widely used for immunological studies, including stem cell research and clinical applications. By providing a reliable method for analyzing and recovering single, viable stem cells based on secretory profile combined with expression of cell surface antigens, this assay format will be useful for stem cell research and clinical applications.

描述(申请人提供)：近年来，很明显，大多数细胞表面标记被许多类型的干细胞和祖细胞所共享。因此，使用当前方法分离独特的细胞亚群的能力是有限的。尽管已知干细胞会将特定的因子分泌到细胞外空间，但很难确定不同种群中单个活细胞的分泌活性。分泌因子(S)与特定细胞类型的关联提供了一个额外的标记，可以用来描述细胞的特征，并在保持细胞活力的同时允许细胞分离。结合免疫表型和分泌谱是研究和最终治疗应用的有效方法。发展新的、可靠的方法来分析和回收单个有活力的干细胞是基于分泌模式结合细胞表面抗原的表达的相当有意义的。GMD干细胞检测满足了对功能性、多参数、单细胞检测的需求，该检测不会影响细胞的活性、生理状态和功能。第一阶段研究优化了检测方法，以用于几个实验性的造血模型。第二阶段研究将扩大该检测方法在包括内皮祖细胞在内的其他模型中的应用。这种检测形式可广泛用于免疫学研究，包括干细胞研究和临床应用。通过提供一种可靠的方法来分析和回收单个有活力的干细胞，该方法结合细胞表面抗原的表达，为干细胞研究和临床应用提供了一种可靠的方法。