New Method for Cell Cryopreservation

细胞冷冻保存新方法

• 批准号: 7404376
• 负责人: Yajuan Jiang
• 金额: $ 9.95万
• 依托单位: ONE CELL SYSTEMS, INC
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-02-01 至 2009-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7404376
• 关键词: Acute monocytic leukemia; Affect; Bathing; Blood Banks; Blood Cells; Blood specimen; Bone Marrow; Bone Marrow Transplantation; Cell Line; Cell Survival; Cells; Child; Count; Cryopreservation; Dimethyl Sulfoxide; Encapsulated; Evaluation; Exhibits; Freezing; Gel; Government; Harvest; High Dose Chemotherapy; Hour; Human; Human Cell Line; Infant; Liquid substance; Malignant Neoplasms; Methodology; Methods; Multiple Myeloma; Neuroblastoma; Neurons; Nitrogen; Numbers; Patients; Peripheral Blood Mononuclear Cell; Peripheral Blood Stem Cell; Peripheral Stem Cell Transplantation; Persons; Phase; Preparation; Process; Purpose; Radiation therapy; Recovery; Research; Sampling; Sepharose; Serum; Staining method; Stains; Stem cell transplant; Stem cells; System; Technology; Time; Transplantation; Trypan Blue; Umbilical Cord Blood; Water; Week; cancer therapy; cell type; day; gel microdrop encapsulation; improved; in vivo; leukemia; leukemia/lymphoma; research study

DESCRIPTION (provided by applicant): Human bone marrow (BM), peripheral blood stem cells (PBSC), and cord blood stem cells (CBSC) are widely used for transplantation in cancer treatment. Patients with leukemia often receive very high doses of chemotherapy and/or radiation therapy which severely damages or destroys bone marrow. Transplantation of BM, PBSC and CBSC can replace stem cells that were destroyed by these treatments and healthy, transplanted stem cells can then restore the ability of bone marrow to produce blood cells in vivo. BM and PBSC are the most frequently used cell types for treating leukemia and lymphoma, and cancers such as multiple myeloma and neuroblastoma (a cancer that arises in immature nerve cells which primarily affects infants and children). Improved methods for sample preparation, and long term storage and cryopreservation of bone marrow and blood samples are urgently needed. After harvesting BM or collecting PBSC and CBSC, samples are processed and frozen to maintain viability until transplantation. Stored blood samples often exhibit problems including cell loss and cell clumping. As the number of transplants continues to increase, research efforts by both public and commercial blood banks aimed at increasing cell viability and recovery after cryopreservation have increased dramatically. Using single cell gel microdrop (GMD) encapsulation technology, this research will develop a method for increasing cell viability and recovery after cryopreservation.

描述（由申请人提供）：人骨髓（BM）、外周血干细胞（PBSC）和脐带血干细胞（CBSC）广泛用于癌症治疗中的移植。白血病患者经常接受非常高剂量的化疗和/或放疗，这会严重损害或破坏骨髓。BM、PBSC和CBSC的移植可以替代被这些治疗破坏的干细胞，然后健康的移植干细胞可以恢复骨髓在体内产生血细胞的能力。BM和PBSC是治疗白血病和淋巴瘤以及癌症如多发性骨髓瘤和神经母细胞瘤（一种主要影响婴儿和儿童的未成熟神经细胞中产生的癌症）最常用的细胞类型。迫切需要用于骨髓和血液样品的样品制备以及长期储存和冷冻保存的改进方法。在收获BM或收集PBSC和CBSC后，处理并冷冻样品以保持活力直至移植。储存的血液样品经常表现出包括细胞损失和细胞凝集的问题。随着移植数量的持续增加，公共和商业血库旨在提高细胞活力和冷冻保存后恢复的研究工作急剧增加。利用单细胞凝胶微滴（GMD）包埋技术，本研究将开发一种提高细胞冻存后存活率和复苏率的方法。