Novel accelerated in vitro breeding for phytoremediation

用于植物修复的新型加速体外育种

• 批准号: 7108052
• 负责人: Mihaly Czako
• 金额: $ 33.96万
• 依托单位: SOUTHERN SUN BIOSYSTEMS, INC.
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-30 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7108052
• 关键词: biotransformation; plant genetics; plant growth /development; plant physiology; technology /technique development

DESCRIPTION (provided by applicant): The long-term goal of the collaboration between the USC and Southern Sun BioSystems is to create a plant breeding and remediation technology-development center which would supply hardware, protocols for breeding-selection and mass production of elite plants for nurseries and remediation businesses, with licensing and tracking mechanisms for protecting intellectual property. This project is built on the successful demonstration of the feasibility of our novel technology for in vitro breeding of a large biomass plant for field-scale phytoremediation. The technology transfer capitalizes on Southern Sun's innovative mass propagation technologies and on somaclonal breeding by amplification of the genetic variation-generating step, that is the number of cell divisions in the totipotent cell cultures subjected to selection. A commercialization plan for elite plant sales and breeding technology is supported by letters of commitment from a variety of remediation and biomass utilizing businesses. Phase II objectives: (1) the frequency and extent of somaclonal variation in second round of culture and (2) preselection using the same pollutant (testing physiological constraints on dehalogenation); (3) add valuable new traits (morphological variations, higher cellulose to lignin ratio) to elite lines from Phase I and (4) to a salt tolerant line; (5) improve mass propagation, transportation, acclimatization by prevention of hyperhydricity; (6) certify elite lines by determining first order detoxification rate constant in hydroponic system, (7) by testing stability of the new traits, and by crop trials to demonstrate remediation of trichlorophenol (and biomass production) of TCP at Southern Sun (8a), and to demonstrate biomass production and remediation of a select pollutant in city sewage sludge at BioTech, Inc., Cayce SC (8b), and potentially in hog lagoons in Clinton, NC. Phase II will produce: elite lines with certified superior haloorganic detoxification ability with added traits to promote end uses, sufficient in vitro stock for mass propagation/field applications, an improved strategic efficiency, cost per unit output, of elite plants for phytoremediation, and pre-booked sales.

描述（由申请人提供）：南加州大学和Southern Sun BioSystems之间合作的长期目标是创建一个植物育种和修复技术开发中心，该中心将为苗圃和修复业务提供硬件，育种选择和大规模生产优质植物的协议，以及保护知识产权的许可和跟踪机制。该项目是建立在我们的新技术的可行性，在体外繁殖的大型生物质植物，现场规模的植物修复的成功示范。技术转让利用了Southern Sun的创新性大规模繁殖技术和体细胞克隆育种，通过扩大遗传变异产生步骤，即经过选择的全能细胞培养物中的细胞分裂次数。精英植物销售和育种技术的商业化计划得到了各种补救和生物质利用企业的承诺书的支持。第二阶段目标：（1）第二轮培养中体细胞无性系变异的频率和程度;（2）使用相同污染物的预选（测试脱卤的生理限制）;（3）增加有价值的新性状（形态变异，较高的纤维素与木质素比率）转化为来自阶段I的优良品系，和（4）转化为耐盐品系;（5）通过防止水分过多，改善大量繁殖、运输和驯化;（6）通过测定水培系统中的一级解毒速率常数来认证优良品系，（7）通过测试新性状的稳定性，以及通过作物试验来证明在Southern Sun（8a）对TCP的三氯苯酚（和生物质生产）的补救，并证明在BioTech，Inc.凯西资深大律师（8b），并可能在猪泻湖克林顿，数控。第二阶段将产生：具有经认证的上级含盐有机解毒能力的优良品系，具有促进最终用途的附加性状，用于大量繁殖/田间应用的足够的体外储备，用于植物修复的优良植物的改进的战略效率、单位产量成本，以及预定销售。